Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26271 | 5' | -53.9 | NC_005345.2 | + | 36372 | 0.66 | 0.776177 |
Target: 5'- gCGUACGCGGGcgcgcuccgcGGCcgCGGcGUGUGGGa -3' miRNA: 3'- -GCGUGCGUCU----------UUGaaGCC-CACGCUCa -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 35749 | 0.66 | 0.765886 |
Target: 5'- uGCgGCGCAGGAcgaACcggCGGG-GCGGGg -3' miRNA: 3'- gCG-UGCGUCUU---UGaa-GCCCaCGCUCa -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 31758 | 0.66 | 0.734202 |
Target: 5'- uGCGgGguGcgaguGCgucUCGGGUGCGGGg -3' miRNA: 3'- gCGUgCguCuu---UGa--AGCCCACGCUCa -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 9963 | 0.66 | 0.734202 |
Target: 5'- uCGCGCGCGGuGAGCUcguguaggcgUCGucauGGUGCGAc- -3' miRNA: 3'- -GCGUGCGUC-UUUGA----------AGC----CCACGCUca -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 23241 | 0.67 | 0.712527 |
Target: 5'- aCGCGCGC-GAGGCcUCgcaggccgagaaGGGggGCGAGUg -3' miRNA: 3'- -GCGUGCGuCUUUGaAG------------CCCa-CGCUCA- -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 30668 | 0.67 | 0.70156 |
Target: 5'- uGCcgaACGCGGcgagcauCUUCGGGUcGCGGGa -3' miRNA: 3'- gCG---UGCGUCuuu----GAAGCCCA-CGCUCa -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 12438 | 0.67 | 0.70156 |
Target: 5'- gCGuCGCGCGGGcuaccucgcgcAGCUcgCGGGgcagGCGAGg -3' miRNA: 3'- -GC-GUGCGUCU-----------UUGAa-GCCCa---CGCUCa -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 35306 | 0.67 | 0.690524 |
Target: 5'- cCGCgaGCGUGGGGACc-CGGGuUGCGGGg -3' miRNA: 3'- -GCG--UGCGUCUUUGaaGCCC-ACGCUCa -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 39592 | 0.67 | 0.690524 |
Target: 5'- gGUGCGCGGGGACUacgaggggcagcUCGGcGUGUGGa- -3' miRNA: 3'- gCGUGCGUCUUUGA------------AGCC-CACGCUca -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 37574 | 0.67 | 0.664937 |
Target: 5'- gCGgGCGCAGGcgGGCUgcaggCGGGcgaccggcucgcguUGCGAGUc -3' miRNA: 3'- -GCgUGCGUCU--UUGAa----GCCC--------------ACGCUCA- -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 31888 | 0.68 | 0.657109 |
Target: 5'- gGUGCGguGAG--UUCGGGgaucgGCGGGUg -3' miRNA: 3'- gCGUGCguCUUugAAGCCCa----CGCUCA- -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 16877 | 0.68 | 0.657109 |
Target: 5'- aCGCGCGCGGGuccgcCUUCGGaaccuugaccGUGCGcGUg -3' miRNA: 3'- -GCGUGCGUCUuu---GAAGCC----------CACGCuCA- -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 38238 | 0.68 | 0.634694 |
Target: 5'- aCGCGCggaccgguGCAGGcgugaucgauGACgugCGGGUGCGGGc -3' miRNA: 3'- -GCGUG--------CGUCU----------UUGaa-GCCCACGCUCa -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 9571 | 0.68 | 0.612268 |
Target: 5'- cCGCaacGCGCGGAAGgUaugCGGGgcgUGCGAGg -3' miRNA: 3'- -GCG---UGCGUCUUUgAa--GCCC---ACGCUCa -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 31448 | 0.69 | 0.601077 |
Target: 5'- aGCGCGgGugcGAGGCgccgGGGUGCGGGUg -3' miRNA: 3'- gCGUGCgU---CUUUGaag-CCCACGCUCA- -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 31194 | 0.69 | 0.589914 |
Target: 5'- gGCGCgGCGGguGCggCGGGUGCGcacccgGGUg -3' miRNA: 3'- gCGUG-CGUCuuUGaaGCCCACGC------UCA- -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 31260 | 0.71 | 0.451006 |
Target: 5'- gCGCACuCGGguGCg-CGGGUGCGGGg -3' miRNA: 3'- -GCGUGcGUCuuUGaaGCCCACGCUCa -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 31943 | 0.72 | 0.402422 |
Target: 5'- gGCGggUGCGGguGCgggugCGGGUGCGGGUg -3' miRNA: 3'- gCGU--GCGUCuuUGaa---GCCCACGCUCA- -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 31720 | 0.74 | 0.335266 |
Target: 5'- gGUGCGCGGAcgggguucgggugcACcUCGGGUGCGGGUg -3' miRNA: 3'- gCGUGCGUCUu-------------UGaAGCCCACGCUCA- -5' |
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26271 | 5' | -53.9 | NC_005345.2 | + | 30870 | 0.84 | 0.070531 |
Target: 5'- aGCGCGCGGguGCUcCGGGUGCGGGc -3' miRNA: 3'- gCGUGCGUCuuUGAaGCCCACGCUCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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