Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26274 | 5' | -56.6 | NC_005345.2 | + | 42856 | 0.66 | 0.588589 |
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Target: 5'- -cCCGGCCGGGaugaaggcggccccGCac-GUGCUGUGGg -3' miRNA: 3'- guGGCUGGCCC--------------CGaaaCACGACACUg -5' |
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| 26274 | 5' | -56.6 | NC_005345.2 | + | 38076 | 0.66 | 0.563406 |
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Target: 5'- gCGCCG-CCGGGcGCcacgcaggcgcagaUUGUGCUGUcGAUg -3' miRNA: 3'- -GUGGCuGGCCC-CGa-------------AACACGACA-CUG- -5' |
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| 26274 | 5' | -56.6 | NC_005345.2 | + | 21744 | 0.67 | 0.548216 |
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Target: 5'- aACCGGCCcgggaGGGCcccUGUGCUGcucgcUGGCg -3' miRNA: 3'- gUGGCUGGc----CCCGaa-ACACGAC-----ACUG- -5' |
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| 26274 | 5' | -56.6 | NC_005345.2 | + | 23943 | 0.67 | 0.543899 |
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Target: 5'- gACCGGCCGGGGgUgauucaccgcggGcUGCUGUcGCg -3' miRNA: 3'- gUGGCUGGCCCCgAaa----------C-ACGACAcUG- -5' |
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| 26274 | 5' | -56.6 | NC_005345.2 | + | 15335 | 0.67 | 0.516131 |
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Target: 5'- -gUCGGCCGGGGCguagcgggUGUGCggcgaaGUaGGCg -3' miRNA: 3'- guGGCUGGCCCCGaa------ACACGa-----CA-CUG- -5' |
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| 26274 | 5' | -56.6 | NC_005345.2 | + | 37784 | 0.68 | 0.464473 |
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Target: 5'- gGCCgcGACCGGGGCUgc-UGCcGUuGGCg -3' miRNA: 3'- gUGG--CUGGCCCCGAaacACGaCA-CUG- -5' |
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| 26274 | 5' | -56.6 | NC_005345.2 | + | 35149 | 0.68 | 0.464472 |
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Target: 5'- gCGgCGAUCGGGGCa-UG-GCUGUGGa -3' miRNA: 3'- -GUgGCUGGCCCCGaaACaCGACACUg -5' |
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| 26274 | 5' | -56.6 | NC_005345.2 | + | 33538 | 1.1 | 0.000513 |
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Target: 5'- cCACCGACCGGGGCUUUGUGCUGUGACg -3' miRNA: 3'- -GUGGCUGGCCCCGAAACACGACACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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