Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26278 | 5' | -58.1 | NC_005345.2 | + | 41843 | 0.72 | 0.249038 |
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Target: 5'- -cCGACUcaUCGGGgaCGACCUCGGcGUc -3' miRNA: 3'- gaGCUGG--AGCCCaaGCUGGAGCC-CAa -5' |
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| 26278 | 5' | -58.1 | NC_005345.2 | + | 9962 | 0.72 | 0.236625 |
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Target: 5'- -gCGGCC-CGGGUUCGGgCUgGGGUc -3' miRNA: 3'- gaGCUGGaGCCCAAGCUgGAgCCCAa -5' |
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| 26278 | 5' | -58.1 | NC_005345.2 | + | 23181 | 0.75 | 0.142922 |
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Target: 5'- -cCGACgcgCUCGGGUUCGACCggcugCGGGa- -3' miRNA: 3'- gaGCUG---GAGCCCAAGCUGGa----GCCCaa -5' |
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| 26278 | 5' | -58.1 | NC_005345.2 | + | 32095 | 0.77 | 0.105526 |
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Target: 5'- -----gCUCGGGUUCGACCUCGGGc- -3' miRNA: 3'- gagcugGAGCCCAAGCUGGAGCCCaa -5' |
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| 26278 | 5' | -58.1 | NC_005345.2 | + | 43354 | 0.8 | 0.073205 |
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Target: 5'- gCUCGACCUCGGGgUCGGCC--GGGUa -3' miRNA: 3'- -GAGCUGGAGCCCaAGCUGGagCCCAa -5' |
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| 26278 | 5' | -58.1 | NC_005345.2 | + | 32071 | 0.92 | 0.008547 |
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Target: 5'- gCUCGACCUCGGGcUCGGCCUCGGGc- -3' miRNA: 3'- -GAGCUGGAGCCCaAGCUGGAGCCCaa -5' |
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| 26278 | 5' | -58.1 | NC_005345.2 | + | 31999 | 0.96 | 0.004891 |
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Target: 5'- cCUCGACCUCGGGUucgaccUCGACCUCGGGUUc -3' miRNA: 3'- -GAGCUGGAGCCCA------AGCUGGAGCCCAA- -5' |
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| 26278 | 5' | -58.1 | NC_005345.2 | + | 32035 | 1.05 | 0.001028 |
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Target: 5'- cCUCGACCUCGGGUUCGACCUCGGGUUc -3' miRNA: 3'- -GAGCUGGAGCCCAAGCUGGAGCCCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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