Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26279 | 5' | -59.4 | NC_005345.2 | + | 30184 | 0.66 | 0.464288 |
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Target: 5'- gACCCaugcggcgagcgCUUCGGacc-GCUGCCGGGCGa -3' miRNA: 3'- -UGGGa-----------GAAGCCacucCGACGGCCUGU- -5' |
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| 26279 | 5' | -59.4 | NC_005345.2 | + | 38032 | 0.66 | 0.427611 |
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Target: 5'- aGCCCg--UCGGUGA-GCUGCgCGG-CGa -3' miRNA: 3'- -UGGGagaAGCCACUcCGACG-GCCuGU- -5' |
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| 26279 | 5' | -59.4 | NC_005345.2 | + | 44653 | 0.67 | 0.418247 |
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Target: 5'- gACCUgCUgCGGUGGgcacGGCUcgucgaGCCGGACAc -3' miRNA: 3'- -UGGGaGAaGCCACU----CCGA------CGGCCUGU- -5' |
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| 26279 | 5' | -59.4 | NC_005345.2 | + | 35829 | 0.68 | 0.364784 |
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Target: 5'- gGCCCgc-UCGGUGGGGUU-CgGGGCAc -3' miRNA: 3'- -UGGGagaAGCCACUCCGAcGgCCUGU- -5' |
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| 26279 | 5' | -59.4 | NC_005345.2 | + | 19986 | 0.68 | 0.364784 |
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Target: 5'- gACCCggUUCGccGAgggcgacaggcgGGCUGCCGGGCAc -3' miRNA: 3'- -UGGGagAAGCcaCU------------CCGACGGCCUGU- -5' |
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| 26279 | 5' | -59.4 | NC_005345.2 | + | 7508 | 0.68 | 0.323977 |
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Target: 5'- cGCCa--UUCGGUGGGcguGCUGCCGGcgGCGg -3' miRNA: 3'- -UGGgagAAGCCACUC---CGACGGCC--UGU- -5' |
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| 26279 | 5' | -59.4 | NC_005345.2 | + | 10374 | 0.69 | 0.316234 |
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Target: 5'- aGCCUUCgagggcgUCGGUGccccacGGCgugaGCCGGGCGa -3' miRNA: 3'- -UGGGAGa------AGCCACu-----CCGa---CGGCCUGU- -5' |
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| 26279 | 5' | -59.4 | NC_005345.2 | + | 7355 | 0.69 | 0.316234 |
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Target: 5'- gGCCCg---CGGUGGcGCaGCCGGGCGg -3' miRNA: 3'- -UGGGagaaGCCACUcCGaCGGCCUGU- -5' |
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| 26279 | 5' | -59.4 | NC_005345.2 | + | 36931 | 0.71 | 0.234144 |
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Target: 5'- cGCCacggC-UCGG-GAGGCggcgGCCGGACAg -3' miRNA: 3'- -UGGga--GaAGCCaCUCCGa---CGGCCUGU- -5' |
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| 26279 | 5' | -59.4 | NC_005345.2 | + | 30485 | 1.08 | 0.000392 |
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Target: 5'- cACCCUCUUCGGUGAGGCUGCCGGACAg -3' miRNA: 3'- -UGGGAGAAGCCACUCCGACGGCCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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