miRNA: 3'- -UugCguUUCgCACCGG---UCUCa---AGUGG- -5'
miRNA: 3'- uUGCGUU--UCGCaCCGG-----UCUCAAGUGG- -5'
miRNA: 3'- -UUGCGUUUCGCACCGGUCUCAAGUGG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2628 | 3' | -52.9 | NC_001491.2 | + | 43090 | 0.73 | 0.693584 |
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Target: 5'- aGugGguGGGgGUGGCCaguAGAGguggUCACCg -3' miRNA: 3'- -UugCguUUCgCACCGG---UCUCa---AGUGG- -5' |
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| 2628 | 3' | -52.9 | NC_001491.2 | + | 31684 | 0.78 | 0.401641 |
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Target: 5'- cGCGCAGccAGCGcGGCCgaagaGGGGUUCACCc -3' miRNA: 3'- uUGCGUU--UCGCaCCGG-----UCUCAAGUGG- -5' |
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| 2628 | 3' | -52.9 | NC_001491.2 | + | 144720 | 1.11 | 0.003638 |
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Target: 5'- gAACGCAAAGCGUGGCCAGAGUUCACCg -3' miRNA: 3'- -UUGCGUUUCGCACCGGUCUCAAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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