Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26284 | 5' | -57.1 | NC_005345.2 | + | 35899 | 0.7 | 0.372397 |
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Target: 5'- ---cUCGCCGGUCGuGCCGacGGUCg- -3' miRNA: 3'- caucAGCGGCCAGCuCGGCa-UCAGgu -5' |
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| 26284 | 5' | -57.1 | NC_005345.2 | + | 6237 | 0.71 | 0.314196 |
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Target: 5'- cUGGUCGCCGGUaCGAacugugugacacgGCCGgugaacgGGUCCc -3' miRNA: 3'- cAUCAGCGGCCA-GCU-------------CGGCa------UCAGGu -5' |
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| 26284 | 5' | -57.1 | NC_005345.2 | + | 8253 | 0.72 | 0.278204 |
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Target: 5'- --cGUCGCCGGguUCGAGCgCGgGGUUCAc -3' miRNA: 3'- cauCAGCGGCC--AGCUCG-GCaUCAGGU- -5' |
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| 26284 | 5' | -57.1 | NC_005345.2 | + | 19347 | 0.73 | 0.244942 |
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Target: 5'- ---cUCGCCGGgggCGAGCgCGUAGUCg- -3' miRNA: 3'- caucAGCGGCCa--GCUCG-GCAUCAGgu -5' |
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| 26284 | 5' | -57.1 | NC_005345.2 | + | 7027 | 0.73 | 0.226614 |
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Target: 5'- -gGGUCGCCGGUCGAccgGCCGgcGaUCgAg -3' miRNA: 3'- caUCAGCGGCCAGCU---CGGCauC-AGgU- -5' |
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| 26284 | 5' | -57.1 | NC_005345.2 | + | 32046 | 0.73 | 0.220768 |
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Target: 5'- aUGGcUGCCGGUCGGGCUGcuGUCCGa -3' miRNA: 3'- cAUCaGCGGCCAGCUCGGCauCAGGU- -5' |
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| 26284 | 5' | -57.1 | NC_005345.2 | + | 44382 | 0.76 | 0.147572 |
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Target: 5'- -gGGUCGCCGG-CGAggguGCCGUcgaGGUCCGa -3' miRNA: 3'- caUCAGCGGCCaGCU----CGGCA---UCAGGU- -5' |
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| 26284 | 5' | -57.1 | NC_005345.2 | + | 26214 | 1.08 | 0.000691 |
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Target: 5'- aGUAGUCGCCGGUCGAGCCGUAGUCCAu -3' miRNA: 3'- -CAUCAGCGGCCAGCUCGGCAUCAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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