miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
26285 3' -54.1 NC_005345.2 + 36181 0.66 0.773217
Target:  5'- aGCGUuggucgcCGGUGGUCCUGcaggCCcugcaaaUGGUGg -3'
miRNA:   3'- -CGCA-------GCCACUAGGACa---GGuag----ACCAC- -5'
26285 3' -54.1 NC_005345.2 + 30759 0.67 0.710966
Target:  5'- gGCGagGGUGAggggUgUGUUCAUCgggGGUGu -3'
miRNA:   3'- -CGCagCCACUa---GgACAGGUAGa--CCAC- -5'
26285 3' -54.1 NC_005345.2 + 29589 0.69 0.582658
Target:  5'- gGUGUCGGUcgagcagcugaacacGGUCCUGUCCucgaUGGg- -3'
miRNA:   3'- -CGCAGCCA---------------CUAGGACAGGuag-ACCac -5'
26285 3' -54.1 NC_005345.2 + 22081 0.76 0.251474
Target:  5'- cGCGUCGGUGAUCUgccCCAUCUGcgccugcGUGg -3'
miRNA:   3'- -CGCAGCCACUAGGacaGGUAGAC-------CAC- -5'
26285 3' -54.1 NC_005345.2 + 25984 1.11 0.000846
Target:  5'- cGCGUCGGUGAUCCUGUCCAUCUGGUGa -3'
miRNA:   3'- -CGCAGCCACUAGGACAGGUAGACCAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.