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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26285 | 3' | -54.1 | NC_005345.2 | + | 36181 | 0.66 | 0.773217 |
Target: 5'- aGCGUuggucgcCGGUGGUCCUGcaggCCcugcaaaUGGUGg -3' miRNA: 3'- -CGCA-------GCCACUAGGACa---GGuag----ACCAC- -5' |
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26285 | 3' | -54.1 | NC_005345.2 | + | 30759 | 0.67 | 0.710966 |
Target: 5'- gGCGagGGUGAggggUgUGUUCAUCgggGGUGu -3' miRNA: 3'- -CGCagCCACUa---GgACAGGUAGa--CCAC- -5' |
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26285 | 3' | -54.1 | NC_005345.2 | + | 29589 | 0.69 | 0.582658 |
Target: 5'- gGUGUCGGUcgagcagcugaacacGGUCCUGUCCucgaUGGg- -3' miRNA: 3'- -CGCAGCCA---------------CUAGGACAGGuag-ACCac -5' |
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26285 | 3' | -54.1 | NC_005345.2 | + | 22081 | 0.76 | 0.251474 |
Target: 5'- cGCGUCGGUGAUCUgccCCAUCUGcgccugcGUGg -3' miRNA: 3'- -CGCAGCCACUAGGacaGGUAGAC-------CAC- -5' |
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26285 | 3' | -54.1 | NC_005345.2 | + | 25984 | 1.11 | 0.000846 |
Target: 5'- cGCGUCGGUGAUCCUGUCCAUCUGGUGa -3' miRNA: 3'- -CGCAGCCACUAGGACAGGUAGACCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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