Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26288 | 5' | -54 | NC_005345.2 | + | 20450 | 0.66 | 0.770991 |
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Target: 5'- cCGUCCGACGCG-GCGUuGUUg---- -3' miRNA: 3'- cGCAGGCUGCGCaCGCAcUAGacuaa -5' |
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| 26288 | 5' | -54 | NC_005345.2 | + | 48420 | 0.66 | 0.75017 |
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Target: 5'- aGCGgcacgUCGACgGCGUGgGcGAUCUGGa- -3' miRNA: 3'- -CGCa----GGCUG-CGCACgCaCUAGACUaa -5' |
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| 26288 | 5' | -54 | NC_005345.2 | + | 17251 | 0.67 | 0.718036 |
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Target: 5'- cGCGUCCGACcggGCGgcagGCGccgcgguccUGGUCUGc-- -3' miRNA: 3'- -CGCAGGCUG---CGCa---CGC---------ACUAGACuaa -5' |
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| 26288 | 5' | -54 | NC_005345.2 | + | 12481 | 0.67 | 0.696154 |
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Target: 5'- cGCGUCCGugcgcACGCGgGCG-GAUCgaGGUUu -3' miRNA: 3'- -CGCAGGC-----UGCGCaCGCaCUAGa-CUAA- -5' |
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| 26288 | 5' | -54 | NC_005345.2 | + | 24020 | 0.67 | 0.696154 |
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Target: 5'- cGCGcucgCCGAgGCGUGUGccGAUgUGGUUg -3' miRNA: 3'- -CGCa---GGCUgCGCACGCa-CUAgACUAA- -5' |
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| 26288 | 5' | -54 | NC_005345.2 | + | 14465 | 0.7 | 0.540691 |
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Target: 5'- uGCGaucgCCGAUGCGUugaGCGUGAUCg---- -3' miRNA: 3'- -CGCa---GGCUGCGCA---CGCACUAGacuaa -5' |
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| 26288 | 5' | -54 | NC_005345.2 | + | 48975 | 0.7 | 0.519102 |
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Target: 5'- uGCGUCUGACGCGUGUGggGAg------ -3' miRNA: 3'- -CGCAGGCUGCGCACGCa-CUagacuaa -5' |
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| 26288 | 5' | -54 | NC_005345.2 | + | 12958 | 0.72 | 0.426918 |
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Target: 5'- cGUGUCCGACGCGcagGCGaGAUCg---- -3' miRNA: 3'- -CGCAGGCUGCGCa--CGCaCUAGacuaa -5' |
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| 26288 | 5' | -54 | NC_005345.2 | + | 24415 | 1.07 | 0.001567 |
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Target: 5'- cGCGUCCGACGCGUGCGUGAUCUGAUUc -3' miRNA: 3'- -CGCAGGCUGCGCACGCACUAGACUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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