miRNA: 3'- -CCAGGA---CUuaCAGCUCC-UGugGGCGc -5'
miRNA: 3'- cCAgGaCUUACAGCUCCUGUGG---GCGC- -5'
miRNA: 3'- -CCAGGacuuaCAGc-UCCUGUGGGCGC- -5'
miRNA: 3'- -CCAGGaCUUACaGCuC-C-UGUGGGCGC- -5'
miRNA: 3'- -CCAGGACUUACAGCUCCUGUGGGCGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26290 | 3' | -55 | NC_005345.2 | + | 34533 | 0.71 | 0.418111 |
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Target: 5'- cGGUCCUcgcGAagGUCGGGGcGCugCUGCu -3' miRNA: 3'- -CCAGGA---CUuaCAGCUCC-UGugGGCGc -5' |
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| 26290 | 3' | -55 | NC_005345.2 | + | 22624 | 0.72 | 0.408771 |
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Target: 5'- cGUgCaGGAUGUCGAGGGCGCCgauCGUGu -3' miRNA: 3'- cCAgGaCUUACAGCUCCUGUGG---GCGC- -5' |
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| 26290 | 3' | -55 | NC_005345.2 | + | 30261 | 0.72 | 0.390488 |
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Target: 5'- gGGUCCggccgGUCacAGGACACCUGCGc -3' miRNA: 3'- -CCAGGacuuaCAGc-UCCUGUGGGCGC- -5' |
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| 26290 | 3' | -55 | NC_005345.2 | + | 31819 | 0.72 | 0.364085 |
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Target: 5'- gGGUCCgGGAUG-CGuGcGuGCACCCGCGg -3' miRNA: 3'- -CCAGGaCUUACaGCuC-C-UGUGGGCGC- -5' |
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| 26290 | 3' | -55 | NC_005345.2 | + | 22964 | 1.12 | 0.000696 |
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Target: 5'- cGGUCCUGAAUGUCGAGGACACCCGCGa -3' miRNA: 3'- -CCAGGACUUACAGCUCCUGUGGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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