Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2630 | 3' | -61.8 | NC_001491.2 | + | 76878 | 0.66 | 0.633166 |
Target: 5'- cACCCCAucGAGUGAGUaGGUCaGGCc-- -3' miRNA: 3'- -UGGGGUu-CUCGCUCG-CCGGcCCGaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 84585 | 0.66 | 0.633166 |
Target: 5'- aGCCCCAAGccGCac-CGGCgGGGCUa- -3' miRNA: 3'- -UGGGGUUCu-CGcucGCCGgCCCGAaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 148702 | 0.66 | 0.623142 |
Target: 5'- cCCCCAuuGGGCG-GCGG-UGGGCa-- -3' miRNA: 3'- uGGGGUu-CUCGCuCGCCgGCCCGaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 67806 | 0.66 | 0.623142 |
Target: 5'- gACUCCcAGGGCG-GCGGCUaucauuuuguuGGGCa-- -3' miRNA: 3'- -UGGGGuUCUCGCuCGCCGG-----------CCCGaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 33699 | 0.66 | 0.603121 |
Target: 5'- cUCCCGAG-GCGcuguGCuccaacccGGCCGGGCUg- -3' miRNA: 3'- uGGGGUUCuCGCu---CG--------CCGGCCCGAaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 12475 | 0.66 | 0.593138 |
Target: 5'- cUCCCGcccGGCGGGCGGC-GGGCg-- -3' miRNA: 3'- uGGGGUuc-UCGCUCGCCGgCCCGaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 3666 | 0.66 | 0.58716 |
Target: 5'- uCCCCGAGcucccugagucugaaGGCGGaCGGCCuGGGCg-- -3' miRNA: 3'- uGGGGUUC---------------UCGCUcGCCGG-CCCGaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 24618 | 0.66 | 0.583182 |
Target: 5'- cGCCCCGGG-GCGGccGCuGCCGcGGCg-- -3' miRNA: 3'- -UGGGGUUCuCGCU--CGcCGGC-CCGaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 10122 | 0.66 | 0.583182 |
Target: 5'- cGCUCC-GGAGCG-GC-GCCGGGCc-- -3' miRNA: 3'- -UGGGGuUCUCGCuCGcCGGCCCGaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 117552 | 0.66 | 0.57326 |
Target: 5'- gACCCCAc-AGCaGAGCGGCCcGGaCUUc -3' miRNA: 3'- -UGGGGUucUCG-CUCGCCGGcCC-GAAa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 2779 | 0.67 | 0.567326 |
Target: 5'- gGCCCCGAGGGaucguaccccCGGGCggacgaggagaaggaGGCCGaGGCUc- -3' miRNA: 3'- -UGGGGUUCUC----------GCUCG---------------CCGGC-CCGAaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 56086 | 0.67 | 0.563378 |
Target: 5'- uGCCCgggggaAAGAGUGcaccgcGGUGGCCGGGUg-- -3' miRNA: 3'- -UGGGg-----UUCUCGC------UCGCCGGCCCGaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 144064 | 0.67 | 0.563378 |
Target: 5'- gUCCUAAGAGCaAGUGGgCGcGGCUUc -3' miRNA: 3'- uGGGGUUCUCGcUCGCCgGC-CCGAAa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 4765 | 0.67 | 0.560422 |
Target: 5'- aGCCCCcgGAGGGaggaggccuccuccCGGGCGGCUGGGa--- -3' miRNA: 3'- -UGGGG--UUCUC--------------GCUCGCCGGCCCgaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 2715 | 0.67 | 0.553542 |
Target: 5'- gGCCCgGGGAGCcuGGUGGCCcgGGGUg-- -3' miRNA: 3'- -UGGGgUUCUCGc-UCGCCGG--CCCGaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 2312 | 0.67 | 0.543757 |
Target: 5'- aGCCgCCGGG-GCGGGaGGCCGGGa--- -3' miRNA: 3'- -UGG-GGUUCuCGCUCgCCGGCCCgaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 34493 | 0.67 | 0.514769 |
Target: 5'- cCCCCAAGcGCaGGGUGGCCaccccGGGCc-- -3' miRNA: 3'- uGGGGUUCuCG-CUCGCCGG-----CCCGaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 94629 | 0.68 | 0.494855 |
Target: 5'- uACCCCAGGGGCGAcacCGGUaggcucacugucuUGGGCg-- -3' miRNA: 3'- -UGGGGUUCUCGCUc--GCCG-------------GCCCGaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 35707 | 0.68 | 0.477145 |
Target: 5'- uCCCCGAgcugcuGAGCGAGUucuGCCGGGUg-- -3' miRNA: 3'- uGGGGUU------CUCGCUCGc--CGGCCCGaaa -5' |
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2630 | 3' | -61.8 | NC_001491.2 | + | 4913 | 0.69 | 0.440932 |
Target: 5'- gGCCCgGgAGAGCcGGCGGCCgacgGGGUUUUc -3' miRNA: 3'- -UGGGgU-UCUCGcUCGCCGG----CCCGAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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