Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2630 | 5' | -56.2 | NC_001491.2 | + | 93717 | 0.66 | 0.918998 |
Target: 5'- cGAGGCUaGCCGUCGcucuauACCgCUagAGGCGc -3' miRNA: 3'- uCUUUGG-CGGCAGC------UGG-GAagUCCGC- -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 140723 | 0.66 | 0.918998 |
Target: 5'- cGcuACCuagguaCGUCGACCUucgaaUUCAGGCGg -3' miRNA: 3'- uCuuUGGcg----GCAGCUGGG-----AAGUCCGC- -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 1344 | 0.66 | 0.913255 |
Target: 5'- cGAGcUCGUCGUCGGgcUCCggCAGGCa -3' miRNA: 3'- uCUUuGGCGGCAGCU--GGGaaGUCCGc -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 19107 | 0.66 | 0.907278 |
Target: 5'- uGGGugCGCUGUUGGCCCggaCAcGGUa -3' miRNA: 3'- uCUUugGCGGCAGCUGGGaa-GU-CCGc -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 131207 | 0.66 | 0.901069 |
Target: 5'- cGGAAAUUGCCGgacCG-CUCUUgAGGUGg -3' miRNA: 3'- -UCUUUGGCGGCa--GCuGGGAAgUCCGC- -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 82239 | 0.67 | 0.881078 |
Target: 5'- -aAAGCCGCUG-CGGCCUUUUuGGUGc -3' miRNA: 3'- ucUUUGGCGGCaGCUGGGAAGuCCGC- -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 8954 | 0.67 | 0.859124 |
Target: 5'- uGGAGCCGCCGagCGACCCcccgaauaccucUUUGGGg- -3' miRNA: 3'- uCUUUGGCGGCa-GCUGGG------------AAGUCCgc -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 5937 | 0.67 | 0.851394 |
Target: 5'- cAGAAGCCGCCcggGUCuGCCCggggaAGGUa -3' miRNA: 3'- -UCUUUGGCGG---CAGcUGGGaag--UCCGc -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 90385 | 0.67 | 0.843468 |
Target: 5'- cGAGGCCgGCC-UCGAacCCCgugaUCAGGUGg -3' miRNA: 3'- uCUUUGG-CGGcAGCU--GGGa---AGUCCGC- -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 12529 | 0.68 | 0.827055 |
Target: 5'- cGGcgGCgGCCGUgGACCgagCGGGCGc -3' miRNA: 3'- -UCuuUGgCGGCAgCUGGgaaGUCCGC- -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 3502 | 0.68 | 0.809943 |
Target: 5'- gGGAGaggGCCGCCGUCuccaGCCCggcCGGGUu -3' miRNA: 3'- -UCUU---UGGCGGCAGc---UGGGaa-GUCCGc -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 130665 | 0.69 | 0.783102 |
Target: 5'- uGGggGgUGCCGguagCGGCCCUUgGGGa- -3' miRNA: 3'- -UCuuUgGCGGCa---GCUGGGAAgUCCgc -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 82177 | 0.69 | 0.773876 |
Target: 5'- cGAGcACCGCCGUCGgACCCgaacUCGacGCGg -3' miRNA: 3'- uCUU-UGGCGGCAGC-UGGGa---AGUc-CGC- -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 28647 | 0.7 | 0.735812 |
Target: 5'- -cGAGCCGCCGggGAgCCUcgCGGGCa -3' miRNA: 3'- ucUUUGGCGGCagCUgGGAa-GUCCGc -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 128975 | 0.76 | 0.405591 |
Target: 5'- uGGAAACCGCCaccGUCGAUgCU-CAGGCu -3' miRNA: 3'- -UCUUUGGCGG---CAGCUGgGAaGUCCGc -5' |
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2630 | 5' | -56.2 | NC_001491.2 | + | 144494 | 1.08 | 0.003493 |
Target: 5'- cAGAAACCGCCGUCGACCCUUCAGGCGc -3' miRNA: 3'- -UCUUUGGCGGCAGCUGGGAAGUCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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