Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26303 | 5' | -51.4 | NC_005345.2 | + | 15809 | 0.66 | 0.887674 |
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Target: 5'- gCGCCGCUCUcGG-CGACUCc------ -3' miRNA: 3'- -GCGGCGAGAaCCaGUUGAGcaacuac -5' |
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| 26303 | 5' | -51.4 | NC_005345.2 | + | 29125 | 0.67 | 0.837196 |
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Target: 5'- gGCUGCUCUgauaGGUCGuCUCGUg---- -3' miRNA: 3'- gCGGCGAGAa---CCAGUuGAGCAacuac -5' |
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| 26303 | 5' | -51.4 | NC_005345.2 | + | 747 | 0.69 | 0.735863 |
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Target: 5'- uGCCGCUCgcGGU--GCUCGUggGAUa -3' miRNA: 3'- gCGGCGAGaaCCAguUGAGCAa-CUAc -5' |
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| 26303 | 5' | -51.4 | NC_005345.2 | + | 6595 | 0.69 | 0.729301 |
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Target: 5'- uCGCUGCUC---GUCGACUCGUgucggacgaugcucgUGAUGu -3' miRNA: 3'- -GCGGCGAGaacCAGUUGAGCA---------------ACUAC- -5' |
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| 26303 | 5' | -51.4 | NC_005345.2 | + | 23237 | 0.69 | 0.702707 |
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Target: 5'- uCGCCGgUCUccgGGUCGuACUCGacGAUGc -3' miRNA: 3'- -GCGGCgAGAa--CCAGU-UGAGCaaCUAC- -5' |
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| 26303 | 5' | -51.4 | NC_005345.2 | + | 29235 | 0.7 | 0.680214 |
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Target: 5'- uCGCUGCUCUUGcGUCGGC-CGaaugccaUGAUGc -3' miRNA: 3'- -GCGGCGAGAAC-CAGUUGaGCa------ACUAC- -5' |
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| 26303 | 5' | -51.4 | NC_005345.2 | + | 11949 | 1.08 | 0.002381 |
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Target: 5'- cCGCCGCUCUUGGUCAACUCGUUGAUGc -3' miRNA: 3'- -GCGGCGAGAACCAGUUGAGCAACUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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