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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2631 | 5' | -46.5 | NC_001491.2 | + | 64967 | 0.66 | 0.999922 |
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Target: 5'- cCGcGcCAGgGCUUGGug--GUUGUCGa -3' miRNA: 3'- -GCaCaGUCgCGAAUCuuuaCAACAGC- -5' |
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| 2631 | 5' | -46.5 | NC_001491.2 | + | 142645 | 0.67 | 0.999666 |
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Target: 5'- aCGUGUCuauaaaaucucacgaGGCGg-UGGAAGcguuUGUUGUCGa -3' miRNA: 3'- -GCACAG---------------UCGCgaAUCUUU----ACAACAGC- -5' |
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| 2631 | 5' | -46.5 | NC_001491.2 | + | 113932 | 0.69 | 0.998079 |
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Target: 5'- gGUGUCAGCGCUgugcuauuacUGUcGUCGu -3' miRNA: 3'- gCACAGUCGCGAaucuuu----ACAaCAGC- -5' |
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| 2631 | 5' | -46.5 | NC_001491.2 | + | 139574 | 0.7 | 0.996669 |
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Target: 5'- --gGUCuGCGCUUuGAccaggcgccgGGUGUUGUCGg -3' miRNA: 3'- gcaCAGuCGCGAAuCU----------UUACAACAGC- -5' |
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| 2631 | 5' | -46.5 | NC_001491.2 | + | 144367 | 1.12 | 0.01668 |
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Target: 5'- cCGUGUCAGCGCUUAGAAAUGUUGUCGg -3' miRNA: 3'- -GCACAGUCGCGAAUCUUUACAACAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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