Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26311 | 5' | -55.8 | NC_005345.2 | + | 23754 | 0.66 | 0.688445 |
Target: 5'- -gCCUCGACGGCgAGGccggcggcgaucGCGUC-GAGc -3' miRNA: 3'- caGGAGCUGCUGgUCC------------UGCAGuCUCc -5' |
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26311 | 5' | -55.8 | NC_005345.2 | + | 36021 | 0.66 | 0.688445 |
Target: 5'- -cCCUUGACGcACgGcGGGCGUUcGAGGu -3' miRNA: 3'- caGGAGCUGC-UGgU-CCUGCAGuCUCC- -5' |
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26311 | 5' | -55.8 | NC_005345.2 | + | 17607 | 0.66 | 0.699186 |
Target: 5'- cUCCUCGcuCGACCcccagcaauGGACGUC-GAGa -3' miRNA: 3'- cAGGAGCu-GCUGGu--------CCUGCAGuCUCc -5' |
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26311 | 5' | -55.8 | NC_005345.2 | + | 29444 | 0.66 | 0.709863 |
Target: 5'- cGUCCUCGGCGuCgAGGcgguacuCGUCGGcccGGu -3' miRNA: 3'- -CAGGAGCUGCuGgUCCu------GCAGUCu--CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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