Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26312 | 5' | -50 | NC_005345.2 | + | 28863 | 0.66 | 0.905583 |
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Target: 5'- aCCgacgucgCCGGCACGaucCGGUUCGUCu -3' miRNA: 3'- aGGaauua--GGUCGUGUa--GUCAGGCAG- -5' |
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| 26312 | 5' | -50 | NC_005345.2 | + | 885 | 0.69 | 0.811424 |
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Target: 5'- ----cGGUCgGGCgGCAUCAGUUCGUCc -3' miRNA: 3'- aggaaUUAGgUCG-UGUAGUCAGGCAG- -5' |
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| 26312 | 5' | -50 | NC_005345.2 | + | 20240 | 0.69 | 0.79118 |
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Target: 5'- cUCCguaucgagAGUCCAGCuCAUCAGUUCa-- -3' miRNA: 3'- -AGGaa------UUAGGUCGuGUAGUCAGGcag -5' |
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| 26312 | 5' | -50 | NC_005345.2 | + | 9510 | 0.69 | 0.79118 |
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Target: 5'- ----cGGUUCAGCGCGUCcGUCuCGUCg -3' miRNA: 3'- aggaaUUAGGUCGUGUAGuCAG-GCAG- -5' |
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| 26312 | 5' | -50 | NC_005345.2 | + | 47068 | 0.69 | 0.780776 |
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Target: 5'- gCCgggacAAUCCuGCACAUCGGUuCCGa- -3' miRNA: 3'- aGGaa---UUAGGuCGUGUAGUCA-GGCag -5' |
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| 26312 | 5' | -50 | NC_005345.2 | + | 9701 | 1.12 | 0.001723 |
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Target: 5'- aUCCUUAAUCCAGCACAUCAGUCCGUCg -3' miRNA: 3'- -AGGAAUUAGGUCGUGUAGUCAGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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