Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26316 | 5' | -55.9 | NC_005345.2 | + | 39554 | 0.7 | 0.404005 |
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Target: 5'- cGGcCCCGCGgccgguccCGGCC-GGUCGUGc -3' miRNA: 3'- -CC-GGGCGCauuu----GUCGGaCCAGCACa -5' |
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| 26316 | 5' | -55.9 | NC_005345.2 | + | 41579 | 0.66 | 0.676796 |
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Target: 5'- uGCUCGCGg--GCGuGCUcGGUCGUGa -3' miRNA: 3'- cCGGGCGCauuUGU-CGGaCCAGCACa -5' |
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| 26316 | 5' | -55.9 | NC_005345.2 | + | 47868 | 0.66 | 0.643779 |
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Target: 5'- gGGCCCGgcgacgGUGAcguCGGCCggcgugcugUGGUCGUGc -3' miRNA: 3'- -CCGGGCg-----CAUUu--GUCGG---------ACCAGCACa -5' |
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| 26316 | 5' | -55.9 | NC_005345.2 | + | 47952 | 0.66 | 0.665818 |
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Target: 5'- uGGCCCGCGggccGGACuGCCgagccgccCGUGUg -3' miRNA: 3'- -CCGGGCGCa---UUUGuCGGacca----GCACA- -5' |
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| 26316 | 5' | -55.9 | NC_005345.2 | + | 48341 | 0.68 | 0.545174 |
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Target: 5'- cGGCCCccgggcgagcagGUGUAAGCgGGCCcGGUCGg-- -3' miRNA: 3'- -CCGGG------------CGCAUUUG-UCGGaCCAGCaca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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