Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2632 | 3' | -43.2 | NC_001491.2 | + | 103211 | 0.66 | 1 |
Target: 5'- aAAAACGguUUACagACAAAGUUcaUGUUCc -3' miRNA: 3'- gUUUUGCguGAUG--UGUUUCAA--ACGAG- -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 19388 | 0.66 | 1 |
Target: 5'- aCAGGACGC-CUcCAUAAAGUUUuuUCu -3' miRNA: 3'- -GUUUUGCGuGAuGUGUUUCAAAcgAG- -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 8783 | 0.66 | 1 |
Target: 5'- ---cGCGCACccUGCGCAGGGcgcUGCUg -3' miRNA: 3'- guuuUGCGUG--AUGUGUUUCaa-ACGAg -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 75194 | 0.66 | 1 |
Target: 5'- gGAGGCuaGaCUACugGCGGAGUUUGCUg -3' miRNA: 3'- gUUUUGcgU-GAUG--UGUUUCAAACGAg -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 95078 | 0.67 | 0.999999 |
Target: 5'- ----uCGUACUGCACAGAGguggguaGCUUu -3' miRNA: 3'- guuuuGCGUGAUGUGUUUCaaa----CGAG- -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 28346 | 0.68 | 0.999996 |
Target: 5'- --uGGCGCGCaGCGgGAAGcucUGCUCg -3' miRNA: 3'- guuUUGCGUGaUGUgUUUCaa-ACGAG- -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 63293 | 0.69 | 0.999978 |
Target: 5'- uCAcgGCGCACUugGCGguguGGGUgaGCUUg -3' miRNA: 3'- -GUuuUGCGUGAugUGU----UUCAaaCGAG- -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 95351 | 0.69 | 0.99997 |
Target: 5'- gAGAGCGC-CUcACACGAugacccauccaGGUUUGCUg -3' miRNA: 3'- gUUUUGCGuGA-UGUGUU-----------UCAAACGAg -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 87674 | 0.69 | 0.999958 |
Target: 5'- uGAAACGCucCUGCGCGuAGcuauugUGCUCu -3' miRNA: 3'- gUUUUGCGu-GAUGUGUuUCaa----ACGAG- -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 123330 | 0.7 | 0.999924 |
Target: 5'- -cAAGCGCGCUccaGCGCGugGAGcuaUGCUCg -3' miRNA: 3'- guUUUGCGUGA---UGUGU--UUCaa-ACGAG- -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 116842 | 0.7 | 0.999898 |
Target: 5'- gCGAGACGCACcgccGCGCGuGGGUUUGUg- -3' miRNA: 3'- -GUUUUGCGUGa---UGUGU-UUCAAACGag -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 28445 | 0.7 | 0.999865 |
Target: 5'- cCAGcAGCGCcCUGCGCAGGGUgcGCg- -3' miRNA: 3'- -GUU-UUGCGuGAUGUGUUUCAaaCGag -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 67728 | 0.7 | 0.999823 |
Target: 5'- --cAGCGCGC-ACACAGAGUccccgUUGCa- -3' miRNA: 3'- guuUUGCGUGaUGUGUUUCA-----AACGag -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 142270 | 0.73 | 0.996897 |
Target: 5'- uGGAGCGCACguuUACAGAGagcaugUGCUCc -3' miRNA: 3'- gUUUUGCGUGau-GUGUUUCaa----ACGAG- -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 118093 | 0.74 | 0.994821 |
Target: 5'- --cGACGCGa-GCGCAAAGUUUGUUUa -3' miRNA: 3'- guuUUGCGUgaUGUGUUUCAAACGAG- -5' |
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2632 | 3' | -43.2 | NC_001491.2 | + | 143566 | 1.11 | 0.035324 |
Target: 5'- uCAAAACGCACUACACAAAGUUUGCUCu -3' miRNA: 3'- -GUUUUGCGUGAUGUGUUUCAAACGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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