Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2632 | 5' | -59 | NC_001491.2 | + | 41481 | 0.66 | 0.796353 |
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Target: 5'- uGAGGCGguuuaUGCGCUGCuGggcuaGGCUCg -3' miRNA: 3'- gCUUCGCagag-ACGCGGCG-Ca----CCGAG- -5' |
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| 2632 | 5' | -59 | NC_001491.2 | + | 81653 | 0.66 | 0.76914 |
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Target: 5'- --cGGUGUUUgUgagccGCGCCGCGggcGGCUCa -3' miRNA: 3'- gcuUCGCAGAgA-----CGCGGCGCa--CCGAG- -5' |
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| 2632 | 5' | -59 | NC_001491.2 | + | 145080 | 0.66 | 0.750394 |
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Target: 5'- cCGAGGCugcgcgGUCUCUGCGCgaCGCGgugaugcgaGGCg- -3' miRNA: 3'- -GCUUCG------CAGAGACGCG--GCGCa--------CCGag -5' |
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| 2632 | 5' | -59 | NC_001491.2 | + | 2619 | 0.67 | 0.721542 |
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Target: 5'- aGccGCGUCgCUGgGCCGCGgagugUGGCa- -3' miRNA: 3'- gCuuCGCAGaGACgCGGCGC-----ACCGag -5' |
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| 2632 | 5' | -59 | NC_001491.2 | + | 64527 | 0.67 | 0.692021 |
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Target: 5'- gGggGUGUCUCcuccgGCcacgGCgGCGUGGCcCa -3' miRNA: 3'- gCuuCGCAGAGa----CG----CGgCGCACCGaG- -5' |
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| 2632 | 5' | -59 | NC_001491.2 | + | 8673 | 0.73 | 0.396029 |
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Target: 5'- aGggGCG-UUCUGCGCCccgcCGUGGCg- -3' miRNA: 3'- gCuuCGCaGAGACGCGGc---GCACCGag -5' |
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| 2632 | 5' | -59 | NC_001491.2 | + | 77360 | 0.76 | 0.271422 |
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Target: 5'- gCGAGGCGUCUUUGUgggcggGCgCGCcUGGCUCg -3' miRNA: 3'- -GCUUCGCAGAGACG------CG-GCGcACCGAG- -5' |
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| 2632 | 5' | -59 | NC_001491.2 | + | 143602 | 1.09 | 0.001482 |
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Target: 5'- gCGAAGCGUCUCUGCGCCGCGUGGCUCu -3' miRNA: 3'- -GCUUCGCAGAGACGCGGCGCACCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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