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miRNA: 3'- gCGAGCCCGCCgCU---UCUUGCAa-AGac -5'
miRNA: 3'- gcGAGC-CCGCCGCUUCUUGCaaagac -5'
miRNA: 3'- gCGagCCCGCCGCuuCUUGCAaaGAc -5'
miRNA: 3'- -GCGAGCCCGCCGCUUCUUGCAAAGAC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 26324 | 5' | -54.2 | NC_005345.2 | + | 35859 | 0.72 | 0.422128 |
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Target: 5'- gGUUCGGGCGGgGGucgGGGGCGUcgUCg- -3' miRNA: 3'- gCGAGCCCGCCgCU---UCUUGCAa-AGac -5' |
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| 26324 | 5' | -54.2 | NC_005345.2 | + | 18955 | 0.73 | 0.367054 |
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Target: 5'- -uCUCGuGGCGGCGAGGAACGa----- -3' miRNA: 3'- gcGAGC-CCGCCGCUUCUUGCaaagac -5' |
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| 26324 | 5' | -54.2 | NC_005345.2 | + | 6545 | 0.75 | 0.258775 |
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Target: 5'- gGCg-GGGCGGUGggGGACGUggCUa -3' miRNA: 3'- gCGagCCCGCCGCuuCUUGCAaaGAc -5' |
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| 26324 | 5' | -54.2 | NC_005345.2 | + | 2852 | 1.08 | 0.001372 |
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Target: 5'- gCGCUCGGGCGGCGAAGAACGUUUCUGu -3' miRNA: 3'- -GCGAGCCCGCCGCUUCUUGCAAAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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