Results 121 - 126 of 126 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26326 | 3' | -57.8 | NC_005345.2 | + | 16366 | 0.77 | 0.102789 |
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Target: 5'- cGGCGCCGCGGUGAucGCGacgaucGGgUUCGUc -3' miRNA: 3'- -CCGCGGCGUCACUuuCGU------CCgGAGCG- -5' |
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| 26326 | 3' | -57.8 | NC_005345.2 | + | 31460 | 0.78 | 0.094177 |
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Target: 5'- aGGCGCCGgGGUGcggguguGAGGCGGGCgagCGCg -3' miRNA: 3'- -CCGCGGCgUCAC-------UUUCGUCCGga-GCG- -5' |
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| 26326 | 3' | -57.8 | NC_005345.2 | + | 23355 | 0.78 | 0.08924 |
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Target: 5'- cGUGCUGCGcUGGGAGCGGGCCgaggCGCc -3' miRNA: 3'- cCGCGGCGUcACUUUCGUCCGGa---GCG- -5' |
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| 26326 | 3' | -57.8 | NC_005345.2 | + | 14196 | 0.79 | 0.081944 |
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Target: 5'- aGGaCGCCGCcgaGGUGuccgccGAGCAGGCCcUCGCc -3' miRNA: 3'- -CC-GCGGCG---UCACu-----UUCGUCCGG-AGCG- -5' |
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| 26326 | 3' | -57.8 | NC_005345.2 | + | 25738 | 0.86 | 0.024938 |
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Target: 5'- gGGCGUCGCGGUGguGGCAGGCgaaccggcucgaCUCGCg -3' miRNA: 3'- -CCGCGGCGUCACuuUCGUCCG------------GAGCG- -5' |
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| 26326 | 3' | -57.8 | NC_005345.2 | + | 2125 | 1.13 | 0.000251 |
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Target: 5'- cGGCGCCGCAGUGAAAGCAGGCCUCGCc -3' miRNA: 3'- -CCGCGGCGUCACUUUCGUCCGGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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