Results 121 - 126 of 126 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26326 | 3' | -57.8 | NC_005345.2 | + | 45977 | 0.7 | 0.337106 |
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Target: 5'- cGCGCCucgcuuucucucuGguGUGguGGCGGGCCcucggggUCGCu -3' miRNA: 3'- cCGCGG-------------CguCACuuUCGUCCGG-------AGCG- -5' |
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| 26326 | 3' | -57.8 | NC_005345.2 | + | 9964 | 0.7 | 0.330666 |
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Target: 5'- cGCG-CGCGGUGAGcucguGUAGGCgUCGUc -3' miRNA: 3'- cCGCgGCGUCACUUu----CGUCCGgAGCG- -5' |
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| 26326 | 3' | -57.8 | NC_005345.2 | + | 27997 | 0.7 | 0.322745 |
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Target: 5'- gGGCGUCGCGuagggcGUcGAGGaugcggagcgcGCAGGCCUCGa -3' miRNA: 3'- -CCGCGGCGU------CA-CUUU-----------CGUCCGGAGCg -5' |
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| 26326 | 3' | -57.8 | NC_005345.2 | + | 44874 | 0.7 | 0.314966 |
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Target: 5'- cGGCgGCCGCGucGcGAGgcgcAGCAacguGGCCUCGCc -3' miRNA: 3'- -CCG-CGGCGU--CaCUU----UCGU----CCGGAGCG- -5' |
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| 26326 | 3' | -57.8 | NC_005345.2 | + | 28972 | 0.7 | 0.314966 |
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Target: 5'- aGGCcccGCCGCAucgGAAugcGGCGGGgCUCGUg -3' miRNA: 3'- -CCG---CGGCGUca-CUU---UCGUCCgGAGCG- -5' |
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| 26326 | 3' | -57.8 | NC_005345.2 | + | 2125 | 1.13 | 0.000251 |
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Target: 5'- cGGCGCCGCAGUGAAAGCAGGCCUCGCc -3' miRNA: 3'- -CCGCGGCGUCACUUUCGUCCGGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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