Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2633 | 3' | -56.5 | NC_001491.2 | + | 36155 | 0.66 | 0.89131 |
Target: 5'- --cUCCCGCCUCcauCCGCUgaCGCUgaacCCCg -3' miRNA: 3'- cacAGGGUGGAGc--GGUGA--GUGAa---GGG- -5' |
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2633 | 3' | -56.5 | NC_001491.2 | + | 3454 | 0.66 | 0.870275 |
Target: 5'- -aGUCCCcgaggaGCCUgGcCCGCUCGaccaggUCCCu -3' miRNA: 3'- caCAGGG------UGGAgC-GGUGAGUga----AGGG- -5' |
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2633 | 3' | -56.5 | NC_001491.2 | + | 109963 | 0.68 | 0.81408 |
Target: 5'- -aGUCCaGCCUCGCCA---GCU-CCCg -3' miRNA: 3'- caCAGGgUGGAGCGGUgagUGAaGGG- -5' |
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2633 | 3' | -56.5 | NC_001491.2 | + | 76241 | 0.69 | 0.727588 |
Target: 5'- --cUCCCGCCgCGCCGCcgccgcagcagcccUCGCU-CCCg -3' miRNA: 3'- cacAGGGUGGaGCGGUG--------------AGUGAaGGG- -5' |
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2633 | 3' | -56.5 | NC_001491.2 | + | 76177 | 0.69 | 0.727588 |
Target: 5'- --cUCCCGCCgCGCCGCcgccgcagcagcccUCGCU-CCCg -3' miRNA: 3'- cacAGGGUGGaGCGGUG--------------AGUGAaGGG- -5' |
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2633 | 3' | -56.5 | NC_001491.2 | + | 76113 | 0.69 | 0.727588 |
Target: 5'- --cUCCCGCCgCGCCGCcgccgcagcagcccUCGCU-CCCg -3' miRNA: 3'- cacAGGGUGGaGCGGUG--------------AGUGAaGGG- -5' |
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2633 | 3' | -56.5 | NC_001491.2 | + | 76049 | 0.69 | 0.727588 |
Target: 5'- --cUCCCGCCgCGCCGCcgccgcagcagcccUCGCU-CCCg -3' miRNA: 3'- cacAGGGUGGaGCGGUG--------------AGUGAaGGG- -5' |
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2633 | 3' | -56.5 | NC_001491.2 | + | 75985 | 0.69 | 0.727588 |
Target: 5'- --cUCCCGCCgCGCCGCcgccgcagcagcccUCGCU-CCCg -3' miRNA: 3'- cacAGGGUGGaGCGGUG--------------AGUGAaGGG- -5' |
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2633 | 3' | -56.5 | NC_001491.2 | + | 84864 | 0.71 | 0.609582 |
Target: 5'- -gGUCCUGCC-CGUCACUCAac-CCCa -3' miRNA: 3'- caCAGGGUGGaGCGGUGAGUgaaGGG- -5' |
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2633 | 3' | -56.5 | NC_001491.2 | + | 33990 | 0.74 | 0.481174 |
Target: 5'- cGUGUCCCugCUgGCCccaccgACUCGg-UCCCa -3' miRNA: 3'- -CACAGGGugGAgCGG------UGAGUgaAGGG- -5' |
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2633 | 3' | -56.5 | NC_001491.2 | + | 142709 | 0.98 | 0.015069 |
Target: 5'- gGUGU-CCACCUCGCCACUCACUUCCCc -3' miRNA: 3'- -CACAgGGUGGAGCGGUGAGUGAAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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