Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26330 | 3' | -58.6 | NC_005345.2 | + | 44686 | 0.66 | 0.481513 |
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Target: 5'- gACAcGGCcgccGGGCCCCCGCCGg----- -3' miRNA: 3'- -UGU-CCGuc--UCUGGGGGCGGCacauac -5' |
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| 26330 | 3' | -58.6 | NC_005345.2 | + | 35003 | 0.66 | 0.481513 |
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Target: 5'- uGCGGGCGGucGGCgaCgCUGCCGUGUGg- -3' miRNA: 3'- -UGUCCGUCu-CUGg-G-GGCGGCACAUac -5' |
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| 26330 | 3' | -58.6 | NC_005345.2 | + | 48595 | 0.66 | 0.481513 |
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Target: 5'- gGCGGGCGaGGACCCaCGCCGg----- -3' miRNA: 3'- -UGUCCGUcUCUGGGgGCGGCacauac -5' |
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| 26330 | 3' | -58.6 | NC_005345.2 | + | 4464 | 0.7 | 0.282427 |
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Target: 5'- --cGGCGGGGGCCCggCgGCCGUGUccGg -3' miRNA: 3'- uguCCGUCUCUGGG--GgCGGCACAuaC- -5' |
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| 26330 | 3' | -58.6 | NC_005345.2 | + | 35195 | 0.7 | 0.26857 |
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Target: 5'- gGCAGGCAGAGggGCgCCCCGuuGcUGUu-- -3' miRNA: 3'- -UGUCCGUCUC--UG-GGGGCggC-ACAuac -5' |
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| 26330 | 3' | -58.6 | NC_005345.2 | + | 11304 | 0.74 | 0.154383 |
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Target: 5'- aGCAGGuCGGAGAUCaaggCCCGCCGUGa--- -3' miRNA: 3'- -UGUCC-GUCUCUGG----GGGCGGCACauac -5' |
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| 26330 | 3' | -58.6 | NC_005345.2 | + | 7956 | 0.75 | 0.134599 |
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Target: 5'- gGCGGGCGGGGAUCCCgucgguCGUCGUGUcGUGc -3' miRNA: 3'- -UGUCCGUCUCUGGGG------GCGGCACA-UAC- -5' |
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| 26330 | 3' | -58.6 | NC_005345.2 | + | 241 | 1.07 | 0.000529 |
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Target: 5'- cACAGGCAGAGACCCCCGCCGUGUAUGu -3' miRNA: 3'- -UGUCCGUCUCUGGGGGCGGCACAUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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