Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26331 | 5' | -50.5 | NC_005345.2 | + | 12189 | 0.7 | 0.743107 |
Target: 5'- aUGg---CAGGGAcucGACGACGAGCGa -3' miRNA: 3'- cACacaaGUUCCUu--CUGCUGCUCGCc -5' |
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26331 | 5' | -50.5 | NC_005345.2 | + | 5772 | 0.7 | 0.732255 |
Target: 5'- uUGUGg-CGAGGGAcGGCGGCGAguucGCGGc -3' miRNA: 3'- cACACaaGUUCCUU-CUGCUGCU----CGCC- -5' |
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26331 | 5' | -50.5 | NC_005345.2 | + | 22855 | 0.73 | 0.56341 |
Target: 5'- ------cCGAGGGugucGACGACGAGCGGg -3' miRNA: 3'- cacacaaGUUCCUu---CUGCUGCUCGCC- -5' |
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26331 | 5' | -50.5 | NC_005345.2 | + | 47424 | 0.74 | 0.541168 |
Target: 5'- ------gCAAGGAAuGCGACGAGCGGu -3' miRNA: 3'- cacacaaGUUCCUUcUGCUGCUCGCC- -5' |
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26331 | 5' | -50.5 | NC_005345.2 | + | 812 | 1.1 | 0.002554 |
Target: 5'- gGUGUGUUCAAGGAAGACGACGAGCGGc -3' miRNA: 3'- -CACACAAGUUCCUUCUGCUGCUCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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