Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26344 | 3' | -62.1 | NC_005345.2 | + | 30691 | 0.66 | 0.314491 |
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Target: 5'- uCGGCguccgGGGCGGUCUGAUcgggcGGCUGCAg -3' miRNA: 3'- -GCCG-----UCCGCCGGGUUGaa---CCGGCGUa -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 16217 | 0.66 | 0.314491 |
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Target: 5'- gGGguGGCGGgggcgucgacgaUCgGGCUcGGCCGCGg -3' miRNA: 3'- gCCguCCGCC------------GGgUUGAaCCGGCGUa -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 38671 | 0.66 | 0.322152 |
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Target: 5'- cCGGC-GGUGGCCgAGgUaaucccGGCCGCGa -3' miRNA: 3'- -GCCGuCCGCCGGgUUgAa-----CCGGCGUa -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 12857 | 0.66 | 0.322152 |
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Target: 5'- cCGGUgacgucguggGGGUGGCUCGccGCcgggUGGCCGCu- -3' miRNA: 3'- -GCCG----------UCCGCCGGGU--UGa---ACCGGCGua -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 31488 | 0.66 | 0.325256 |
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Target: 5'- gCGGaucGGCGGCCCcgcgaacacggucguGACcgaccGGCCGCGUc -3' miRNA: 3'- -GCCgu-CCGCCGGG---------------UUGaa---CCGGCGUA- -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 8591 | 0.66 | 0.329953 |
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Target: 5'- aCGGUcgAGGCGGCCCGuccACUgccagUGGaCGCc- -3' miRNA: 3'- -GCCG--UCCGCCGGGU---UGA-----ACCgGCGua -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 6831 | 0.66 | 0.329953 |
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Target: 5'- uGGCGGGCGGgaUCGACaUGGUccuCGCGg -3' miRNA: 3'- gCCGUCCGCCg-GGUUGaACCG---GCGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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