Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26344 | 3' | -62.1 | NC_005345.2 | + | 11444 | 0.74 | 0.08824 |
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Target: 5'- -cGCAGGCGGCCgCGACcggGGCUGCu- -3' miRNA: 3'- gcCGUCCGCCGG-GUUGaa-CCGGCGua -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 44758 | 0.75 | 0.072471 |
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Target: 5'- cCGGCGGG-GGCCCGGC--GGCCGUGUc -3' miRNA: 3'- -GCCGUCCgCCGGGUUGaaCCGGCGUA- -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 2044 | 0.76 | 0.061142 |
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Target: 5'- gGGCccGGcGCGGCCCGGCg-GGCCGCGg -3' miRNA: 3'- gCCG--UC-CGCCGGGUUGaaCCGGCGUa -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 8684 | 0.77 | 0.051094 |
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Target: 5'- gCGGCgguggccgugacguGGGCGGCCCGGCUgUGGCCGa-- -3' miRNA: 3'- -GCCG--------------UCCGCCGGGUUGA-ACCGGCgua -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 42872 | 0.77 | 0.044664 |
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Target: 5'- aCGGCAcGaGCGGCgCCGGgUUGGCCGCGg -3' miRNA: 3'- -GCCGU-C-CGCCG-GGUUgAACCGGCGUa -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 32518 | 0.81 | 0.021708 |
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Target: 5'- cCGGCGGGCGGCCCGAuCUgcgcggguccgGGCCGCc- -3' miRNA: 3'- -GCCGUCCGCCGGGUU-GAa----------CCGGCGua -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 6340 | 1.05 | 0.000321 |
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Target: 5'- gCGGCAGGCGGCCCAACUUGGCCGCAUc -3' miRNA: 3'- -GCCGUCCGCCGGGUUGAACCGGCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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