Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26344 | 3' | -62.1 | NC_005345.2 | + | 19099 | 0.68 | 0.233222 |
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Target: 5'- aGGCGGagcaGCGGCCCGAg--GGCgGCGc -3' miRNA: 3'- gCCGUC----CGCCGGGUUgaaCCGgCGUa -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 3649 | 0.68 | 0.233222 |
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Target: 5'- gGGCGGGcCGGUUgCGGCcgggGGCCGCGa -3' miRNA: 3'- gCCGUCC-GCCGG-GUUGaa--CCGGCGUa -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 9146 | 0.68 | 0.23926 |
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Target: 5'- cCGGCGacGGCGGCCCGGauaaaGUCGCGg -3' miRNA: 3'- -GCCGU--CCGCCGGGUUgaac-CGGCGUa -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 48041 | 0.67 | 0.245427 |
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Target: 5'- gCGGCgcacacGGGCGGCUCGGCaguccGGcCCGCGg -3' miRNA: 3'- -GCCG------UCCGCCGGGUUGaa---CC-GGCGUa -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 36209 | 0.67 | 0.251726 |
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Target: 5'- uCGGC-GGCcccugaGGaCCCGccgaagagcgACUUGGCCGCGUc -3' miRNA: 3'- -GCCGuCCG------CC-GGGU----------UGAACCGGCGUA- -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 13376 | 0.67 | 0.258157 |
|
Target: 5'- gCGGCGGGUGGCUCggUUcgGGCgGgGg -3' miRNA: 3'- -GCCGUCCGCCGGGuuGAa-CCGgCgUa -5' |
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| 26344 | 3' | -62.1 | NC_005345.2 | + | 8591 | 0.66 | 0.329953 |
|
Target: 5'- aCGGUcgAGGCGGCCCGuccACUgccagUGGaCGCc- -3' miRNA: 3'- -GCCG--UCCGCCGGGU---UGA-----ACCgGCGua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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