Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2635 | 3' | -59.5 | NC_001491.2 | + | 3353 | 0.66 | 0.786902 |
Target: 5'- -gGCGUuguaGCGcACCAGCGGCGccacgGuCCGg -3' miRNA: 3'- ugCGCG----UGC-UGGUCGCCGCaua--C-GGC- -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 12581 | 0.66 | 0.784212 |
Target: 5'- gGCGCGCauccccccccuccgACGGCCGccgccGCGGCagcgGCCGc -3' miRNA: 3'- -UGCGCG--------------UGCUGGU-----CGCCGcauaCGGC- -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 10367 | 0.66 | 0.776982 |
Target: 5'- uCGCGCcCGcagauguACgAGCGcGCGUGUGCgCGg -3' miRNA: 3'- uGCGCGuGC-------UGgUCGC-CGCAUACG-GC- -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 14554 | 0.66 | 0.768754 |
Target: 5'- cCGCGCACGugcagauaauaACCAGCaaaaaGGCGagguaGUGUCGa -3' miRNA: 3'- uGCGCGUGC-----------UGGUCG-----CCGCa----UACGGC- -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 3830 | 0.66 | 0.768754 |
Target: 5'- -gGC-CGCGG-CAGCGGCGgccuggGCCGg -3' miRNA: 3'- ugCGcGUGCUgGUCGCCGCaua---CGGC- -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 118125 | 0.66 | 0.750143 |
Target: 5'- uUGUGUACaGCCAGCGGU-UAcGCCGg -3' miRNA: 3'- uGCGCGUGcUGGUCGCCGcAUaCGGC- -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 2622 | 0.67 | 0.721506 |
Target: 5'- cGCGuCGCugGGCC-GCGGaGUGUGgCa -3' miRNA: 3'- -UGC-GCGugCUGGuCGCCgCAUACgGc -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 127811 | 0.67 | 0.721506 |
Target: 5'- -gGCGgACGGCCGGCauuuacguguGGUccuuGUAUGCCa -3' miRNA: 3'- ugCGCgUGCUGGUCG----------CCG----CAUACGGc -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 145508 | 0.67 | 0.711803 |
Target: 5'- gACGCGCACa---AGCGGCG-AUGCa- -3' miRNA: 3'- -UGCGCGUGcuggUCGCCGCaUACGgc -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 90644 | 0.67 | 0.711803 |
Target: 5'- -gGCGCAUG-CgAGUGGUGUuUGCUGa -3' miRNA: 3'- ugCGCGUGCuGgUCGCCGCAuACGGC- -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 54079 | 0.67 | 0.710829 |
Target: 5'- cCGCGCAgCuGCUuuaucucuuuccuAGCGGCGUAUGCg- -3' miRNA: 3'- uGCGCGU-GcUGG-------------UCGCCGCAUACGgc -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 26797 | 0.67 | 0.702035 |
Target: 5'- -aGCGCucuGCGuccGCCGGCGGCGUcuccccgaucccGaGCCGg -3' miRNA: 3'- ugCGCG---UGC---UGGUCGCCGCA------------UaCGGC- -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 74715 | 0.68 | 0.662486 |
Target: 5'- gGCGaguCGCGUGGCCAGCGGCGcAUcGCUa -3' miRNA: 3'- -UGC---GCGUGCUGGUCGCCGCaUA-CGGc -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 8464 | 0.68 | 0.662486 |
Target: 5'- uGC-CGCAUGGcCCAGCGGgGgacGCCGu -3' miRNA: 3'- -UGcGCGUGCU-GGUCGCCgCauaCGGC- -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 136295 | 0.68 | 0.65252 |
Target: 5'- aGCGCGCcggggcugGCGGCCuugGGCGGUGgcagcGCCa -3' miRNA: 3'- -UGCGCG--------UGCUGG---UCGCCGCaua--CGGc -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 11472 | 0.68 | 0.63255 |
Target: 5'- gUGCGCGCGGCU-GCGG-GgcUGCCu -3' miRNA: 3'- uGCGCGUGCUGGuCGCCgCauACGGc -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 118393 | 0.68 | 0.622561 |
Target: 5'- gUGgGCACcaGACCuGCGGgGUcgGCCa -3' miRNA: 3'- uGCgCGUG--CUGGuCGCCgCAuaCGGc -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 32184 | 0.68 | 0.622561 |
Target: 5'- gACGaGgACGACCAgGCGGaugGUGCCGg -3' miRNA: 3'- -UGCgCgUGCUGGU-CGCCgcaUACGGC- -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 81484 | 0.69 | 0.582743 |
Target: 5'- gAUGcCGCGCGugCAGCGGagagcGCCGa -3' miRNA: 3'- -UGC-GCGUGCugGUCGCCgcauaCGGC- -5' |
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2635 | 3' | -59.5 | NC_001491.2 | + | 26714 | 0.69 | 0.572857 |
Target: 5'- gGCGCGCAgGuacGCC-GCGGCGgcUGCg- -3' miRNA: 3'- -UGCGCGUgC---UGGuCGCCGCauACGgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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