Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2635 | 3' | -59.5 | NC_001491.2 | + | 74715 | 0.68 | 0.662486 |
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Target: 5'- gGCGaguCGCGUGGCCAGCGGCGcAUcGCUa -3' miRNA: 3'- -UGC---GCGUGCUGGUCGCCGCaUA-CGGc -5' |
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| 2635 | 3' | -59.5 | NC_001491.2 | + | 81484 | 0.69 | 0.582743 |
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Target: 5'- gAUGcCGCGCGugCAGCGGagagcGCCGa -3' miRNA: 3'- -UGC-GCGUGCugGUCGCCgcauaCGGC- -5' |
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| 2635 | 3' | -59.5 | NC_001491.2 | + | 90644 | 0.67 | 0.711803 |
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Target: 5'- -gGCGCAUG-CgAGUGGUGUuUGCUGa -3' miRNA: 3'- ugCGCGUGCuGgUCGCCGCAuACGGC- -5' |
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| 2635 | 3' | -59.5 | NC_001491.2 | + | 118125 | 0.66 | 0.750143 |
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Target: 5'- uUGUGUACaGCCAGCGGU-UAcGCCGg -3' miRNA: 3'- uGCGCGUGcUGGUCGCCGcAUaCGGC- -5' |
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| 2635 | 3' | -59.5 | NC_001491.2 | + | 118393 | 0.68 | 0.622561 |
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Target: 5'- gUGgGCACcaGACCuGCGGgGUcgGCCa -3' miRNA: 3'- uGCgCGUG--CUGGuCGCCgCAuaCGGc -5' |
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| 2635 | 3' | -59.5 | NC_001491.2 | + | 127811 | 0.67 | 0.721506 |
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Target: 5'- -gGCGgACGGCCGGCauuuacguguGGUccuuGUAUGCCa -3' miRNA: 3'- ugCGCgUGCUGGUCG----------CCG----CAUACGGc -5' |
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| 2635 | 3' | -59.5 | NC_001491.2 | + | 136295 | 0.68 | 0.65252 |
|
Target: 5'- aGCGCGCcggggcugGCGGCCuugGGCGGUGgcagcGCCa -3' miRNA: 3'- -UGCGCG--------UGCUGG---UCGCCGCaua--CGGc -5' |
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| 2635 | 3' | -59.5 | NC_001491.2 | + | 141615 | 1.07 | 0.001787 |
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Target: 5'- aACGCGCACGACCAGCGGCGUAUGCCGu -3' miRNA: 3'- -UGCGCGUGCUGGUCGCCGCAUACGGC- -5' |
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| 2635 | 3' | -59.5 | NC_001491.2 | + | 145508 | 0.67 | 0.711803 |
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Target: 5'- gACGCGCACa---AGCGGCG-AUGCa- -3' miRNA: 3'- -UGCGCGUGcuggUCGCCGCaUACGgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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