Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26355 | 5' | -56.8 | NC_005345.2 | + | 39596 | 0.66 | 0.582844 |
Target: 5'- cGCGGGGacuacgagGGGcAGCUCGgcgUGUGg- -3' miRNA: 3'- -CGCCCCca------CCC-UCGAGCaaaACGCaa -5' |
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26355 | 5' | -56.8 | NC_005345.2 | + | 33362 | 0.67 | 0.539378 |
Target: 5'- cGCGagaGGGGUGGGGGCg-GUggcGCGg- -3' miRNA: 3'- -CGC---CCCCACCCUCGagCAaaaCGCaa -5' |
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26355 | 5' | -56.8 | NC_005345.2 | + | 29583 | 0.67 | 0.51805 |
Target: 5'- gGCGGGguccuuccaguGGUGGGAGaUCGg---GCGUa -3' miRNA: 3'- -CGCCC-----------CCACCCUCgAGCaaaaCGCAa -5' |
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26355 | 5' | -56.8 | NC_005345.2 | + | 35865 | 0.7 | 0.363055 |
Target: 5'- gGCGGGGGUcGGGGGCgUCGUc--GCc-- -3' miRNA: 3'- -CGCCCCCA-CCCUCG-AGCAaaaCGcaa -5' |
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26355 | 5' | -56.8 | NC_005345.2 | + | 33904 | 0.72 | 0.249688 |
Target: 5'- -aGGGGGUGGGAGCUC----UGCc-- -3' miRNA: 3'- cgCCCCCACCCUCGAGcaaaACGcaa -5' |
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26355 | 5' | -56.8 | NC_005345.2 | + | 15276 | 1.06 | 0.000864 |
Target: 5'- gGCGGGGGUGGGAGCUCGUUUUGCGUUc -3' miRNA: 3'- -CGCCCCCACCCUCGAGCAAAACGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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