Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2637 | 3' | -54.4 | NC_001491.2 | + | 12493 | 0.66 | 0.956268 |
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Target: 5'- cGGgcgcccgCGGGUCCCcgggGCGGgGCGGcguCGCGg -3' miRNA: 3'- -CCaa-----GUUCAGGG----UGUCaCGCCu--GCGC- -5' |
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| 2637 | 3' | -54.4 | NC_001491.2 | + | 30173 | 0.66 | 0.943779 |
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Target: 5'- gGGUUaacacaCGAGggcgCCCuc-GUGCGGACGUGu -3' miRNA: 3'- -CCAA------GUUCa---GGGuguCACGCCUGCGC- -5' |
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| 2637 | 3' | -54.4 | NC_001491.2 | + | 103970 | 0.67 | 0.939148 |
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Target: 5'- aGGUgcuggGGGUUCUugAG-GCGGugGCGu -3' miRNA: 3'- -CCAag---UUCAGGGugUCaCGCCugCGC- -5' |
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| 2637 | 3' | -54.4 | NC_001491.2 | + | 5739 | 0.67 | 0.918227 |
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Target: 5'- cGGUggcgGGGUUCCGCg--GCGGGCGCu -3' miRNA: 3'- -CCAag--UUCAGGGUGucaCGCCUGCGc -5' |
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| 2637 | 3' | -54.4 | NC_001491.2 | + | 32006 | 0.68 | 0.886722 |
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Target: 5'- --cUCAucaCCCgGCGGUGgGGACGCGg -3' miRNA: 3'- ccaAGUucaGGG-UGUCACgCCUGCGC- -5' |
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| 2637 | 3' | -54.4 | NC_001491.2 | + | 28328 | 0.68 | 0.886722 |
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Target: 5'- aGUUC-AGUgCCCGCAGcGUGG-CGCGc -3' miRNA: 3'- cCAAGuUCA-GGGUGUCaCGCCuGCGC- -5' |
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| 2637 | 3' | -54.4 | NC_001491.2 | + | 35166 | 0.7 | 0.798613 |
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Target: 5'- aGGcgCAAGcUCCU--GGUGCuGGACGCGg -3' miRNA: 3'- -CCaaGUUC-AGGGugUCACG-CCUGCGC- -5' |
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| 2637 | 3' | -54.4 | NC_001491.2 | + | 142896 | 0.71 | 0.761494 |
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Target: 5'- cGUUUcuGUCCauaAGUGCGGACGUGc -3' miRNA: 3'- cCAAGuuCAGGgugUCACGCCUGCGC- -5' |
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| 2637 | 3' | -54.4 | NC_001491.2 | + | 136863 | 1.12 | 0.00293 |
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Target: 5'- gGGUUCAAGUCCCACAGUGCGGACGCGg -3' miRNA: 3'- -CCAAGUUCAGGGUGUCACGCCUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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