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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 2637 | 5' | -59.7 | NC_001491.2 | + | 143596 | 0.66 | 0.755228 |
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Target: 5'- uGC-UGUGCGaaGCGucucugcGCCGCGUGGCUCu -3' miRNA: 3'- gCGcACACGC--CGU-------CGGUGCGCUGAGu -5' |
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| 2637 | 5' | -59.7 | NC_001491.2 | + | 90144 | 0.66 | 0.73725 |
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Target: 5'- aGCGcGUGCGGUAgguGCCGCGCaAUgUCAa -3' miRNA: 3'- gCGCaCACGCCGU---CGGUGCGcUG-AGU- -5' |
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| 2637 | 5' | -59.7 | NC_001491.2 | + | 3724 | 0.67 | 0.717987 |
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Target: 5'- cCGCGgccGCGGCAGCCuuGCGuCg-- -3' miRNA: 3'- -GCGCacaCGCCGUCGGugCGCuGagu -5' |
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| 2637 | 5' | -59.7 | NC_001491.2 | + | 93696 | 0.67 | 0.668749 |
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Target: 5'- gGC-UGUGuCGGCAGCCAUGaccgaGGCUa- -3' miRNA: 3'- gCGcACAC-GCCGUCGGUGCg----CUGAgu -5' |
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| 2637 | 5' | -59.7 | NC_001491.2 | + | 110022 | 0.69 | 0.578953 |
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Target: 5'- aCGUGUuugGCGGCGGCUAuCGCGGCc-- -3' miRNA: 3'- -GCGCAca-CGCCGUCGGU-GCGCUGagu -5' |
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| 2637 | 5' | -59.7 | NC_001491.2 | + | 26715 | 0.69 | 0.588875 |
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Target: 5'- gCGCGcagGUacgccGCGGCGGCUGCGUGGCg-- -3' miRNA: 3'- -GCGCa--CA-----CGCCGUCGGUGCGCUGagu -5' |
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| 2637 | 5' | -59.7 | NC_001491.2 | + | 129508 | 0.69 | 0.598827 |
|
Target: 5'- gGUGUGUGCuauGCAGCC-CGCccACUCAg -3' miRNA: 3'- gCGCACACGc--CGUCGGuGCGc-UGAGU- -5' |
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| 2637 | 5' | -59.7 | NC_001491.2 | + | 44171 | 0.72 | 0.39476 |
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Target: 5'- aGCGcG-GCGGCAGCCGCgGCGaggccaaccacGCUCAg -3' miRNA: 3'- gCGCaCaCGCCGUCGGUG-CGC-----------UGAGU- -5' |
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| 2637 | 5' | -59.7 | NC_001491.2 | + | 69105 | 0.74 | 0.317896 |
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Target: 5'- uGCGUaGUGCGGCGGCUGCGUaGGCg-- -3' miRNA: 3'- gCGCA-CACGCCGUCGGUGCG-CUGagu -5' |
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| 2637 | 5' | -59.7 | NC_001491.2 | + | 136897 | 1.08 | 0.001616 |
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Target: 5'- uCGCGUGUGCGGCAGCCACGCGACUCAc -3' miRNA: 3'- -GCGCACACGCCGUCGGUGCGCUGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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