Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2638 | 3' | -60.4 | NC_001491.2 | + | 8782 | 0.66 | 0.72416 |
Target: 5'- uCGCGcaccCUG-CGCagGGCGCUGCUGg -3' miRNA: 3'- -GCGCuuc-GACgGCGaaCCGCGACGGC- -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 25300 | 0.66 | 0.714449 |
Target: 5'- aGCGAGG-UGCCGUgccGCG-UGCCGg -3' miRNA: 3'- gCGCUUCgACGGCGaacCGCgACGGC- -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 3181 | 0.66 | 0.709568 |
Target: 5'- cCGCGGAGCcggagaagggcucGCCGCcgGGCgaggacgaugaggGCUGCUGg -3' miRNA: 3'- -GCGCUUCGa------------CGGCGaaCCG-------------CGACGGC- -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 115312 | 0.66 | 0.688907 |
Target: 5'- gCGCGgcGCgUGCCGCUgagauguaguucauaUacgGGCGCggUGCCa -3' miRNA: 3'- -GCGCuuCG-ACGGCGA---------------A---CCGCG--ACGGc -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 119207 | 0.67 | 0.665055 |
Target: 5'- cCGCG-AGCUGUgCGCccUGGgGCUGCa- -3' miRNA: 3'- -GCGCuUCGACG-GCGa-ACCgCGACGgc -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 86345 | 0.67 | 0.655067 |
Target: 5'- gCGUGGAGCUgGuuGC--GGCGCUGUa- -3' miRNA: 3'- -GCGCUUCGA-CggCGaaCCGCGACGgc -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 2975 | 0.67 | 0.645062 |
Target: 5'- aGCGGGccuuCUGCgGCUgGGCuGCUGCCc -3' miRNA: 3'- gCGCUUc---GACGgCGAaCCG-CGACGGc -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 35755 | 0.67 | 0.625029 |
Target: 5'- aCGcCGAGGCcgaGCC-CcUGGUGCUGCCc -3' miRNA: 3'- -GC-GCUUCGa--CGGcGaACCGCGACGGc -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 20442 | 0.68 | 0.575159 |
Target: 5'- cCGCGGuggucGUUGCUGCUgUGGuCGUUGCUGc -3' miRNA: 3'- -GCGCUu----CGACGGCGA-ACC-GCGACGGC- -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 5743 | 0.68 | 0.575159 |
Target: 5'- gGCGggGUU-CCGCggcgGGCGCUcggacgacgggcGCCGc -3' miRNA: 3'- gCGCuuCGAcGGCGaa--CCGCGA------------CGGC- -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 10047 | 0.68 | 0.565275 |
Target: 5'- aCGCGGucucGCUGCCGCgcuccgUGGgGgacuUUGCCGc -3' miRNA: 3'- -GCGCUu---CGACGGCGa-----ACCgC----GACGGC- -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 24626 | 0.69 | 0.535917 |
Target: 5'- gGCGGccGCUGCCGC--GGCgGCgGCCGu -3' miRNA: 3'- gCGCUu-CGACGGCGaaCCG-CGaCGGC- -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 4449 | 0.69 | 0.497652 |
Target: 5'- -aCGAAGCcGCCGCcggGGCcgaugcugcuGCUGCCGc -3' miRNA: 3'- gcGCUUCGaCGGCGaa-CCG----------CGACGGC- -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 66461 | 0.7 | 0.488275 |
Target: 5'- cCGCGGAGCUGauaGCUaGGUGUguUGCCc -3' miRNA: 3'- -GCGCUUCGACgg-CGAaCCGCG--ACGGc -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 137043 | 0.7 | 0.451641 |
Target: 5'- gGCGAGGCgcGCUGUUUGGgagcgcaguccUGUUGCCGg -3' miRNA: 3'- gCGCUUCGa-CGGCGAACC-----------GCGACGGC- -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 128554 | 0.7 | 0.451641 |
Target: 5'- uGCGggGCcGCgGCacUGGCGCUGgUCGu -3' miRNA: 3'- gCGCuuCGaCGgCGa-ACCGCGAC-GGC- -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 32382 | 0.71 | 0.399658 |
Target: 5'- cCGaaGAGCUGCCGCUccGGCGCcGCUu -3' miRNA: 3'- -GCgcUUCGACGGCGAa-CCGCGaCGGc -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 33508 | 0.72 | 0.383199 |
Target: 5'- aCGCaAGGCUGCCGC--GGCcGCgGCCGc -3' miRNA: 3'- -GCGcUUCGACGGCGaaCCG-CGaCGGC- -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 133944 | 0.72 | 0.383199 |
Target: 5'- gCGCGAuAGCUguaccaggcGCCGagagGGCGUUGCCGu -3' miRNA: 3'- -GCGCU-UCGA---------CGGCgaa-CCGCGACGGC- -5' |
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2638 | 3' | -60.4 | NC_001491.2 | + | 35721 | 0.8 | 0.116464 |
Target: 5'- aGCGAGuuCUGCCGggUGGCGCUGCUGg -3' miRNA: 3'- gCGCUUc-GACGGCgaACCGCGACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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