Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2638 | 5' | -52.5 | NC_001491.2 | + | 32198 | 0.66 | 0.97307 |
Target: 5'- gGCgGAUGGUGCcgGgGCgAGUAGCUCCUc -3' miRNA: 3'- aCG-UUAUCGCGa-UgCG-UUAUCGGGGA- -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 16845 | 0.66 | 0.97307 |
Target: 5'- cUGCGGuUGGCGCguggucaaguuuUACGCGAaggauaUAcGCCCCg -3' miRNA: 3'- -ACGUU-AUCGCG------------AUGCGUU------AU-CGGGGa -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 60981 | 0.66 | 0.966971 |
Target: 5'- cUGCGcgAGCGCUcugGCGUuuUGuucuacucGCCCCa -3' miRNA: 3'- -ACGUuaUCGCGA---UGCGuuAU--------CGGGGa -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 81494 | 0.66 | 0.966971 |
Target: 5'- gUGCAGcggagAGCGCcgACGCcgccacGCCCCa -3' miRNA: 3'- -ACGUUa----UCGCGa-UGCGuuau--CGGGGa -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 20260 | 0.66 | 0.963581 |
Target: 5'- gGCAGUGGaaGCUgaggggguggACGUGGaAGCCCCUg -3' miRNA: 3'- aCGUUAUCg-CGA----------UGCGUUaUCGGGGA- -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 65163 | 0.66 | 0.959956 |
Target: 5'- cGaCAGaggAGUGCUACGCGcuuggaaucGGCCCCg -3' miRNA: 3'- aC-GUUa--UCGCGAUGCGUua-------UCGGGGa -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 33467 | 0.66 | 0.959956 |
Target: 5'- cGCGA-AGCGUacacccgggucUGCGCGGcccucgGGCCCCg -3' miRNA: 3'- aCGUUaUCGCG-----------AUGCGUUa-----UCGGGGa -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 82743 | 0.67 | 0.956089 |
Target: 5'- gUGCuugccGCGCacgACGCcGUAGCCCUc -3' miRNA: 3'- -ACGuuau-CGCGa--UGCGuUAUCGGGGa -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 91403 | 0.67 | 0.951976 |
Target: 5'- gGCca-GGUGCUGCGUGcgGGCuCCCa -3' miRNA: 3'- aCGuuaUCGCGAUGCGUuaUCG-GGGa -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 66906 | 0.67 | 0.951976 |
Target: 5'- cGCAAcGGCGCgucggACGauauguuGGUGGCCCUg -3' miRNA: 3'- aCGUUaUCGCGa----UGCg------UUAUCGGGGa -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 28555 | 0.67 | 0.942993 |
Target: 5'- cGCcacGGCGggGCGCAGaacGCCCCUg -3' miRNA: 3'- aCGuuaUCGCgaUGCGUUau-CGGGGA- -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 11758 | 0.68 | 0.932983 |
Target: 5'- aUGUAAUAGCGCUcgucCGCG--GGUUCCUc -3' miRNA: 3'- -ACGUUAUCGCGAu---GCGUuaUCGGGGA- -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 32956 | 0.68 | 0.932983 |
Target: 5'- cGCcuc-GCGCUACGCcGAggcgcaGGCCCCa -3' miRNA: 3'- aCGuuauCGCGAUGCG-UUa-----UCGGGGa -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 35269 | 0.68 | 0.932983 |
Target: 5'- cGCGGgucgccuGCGCcgUGCGC--UGGCCCCg -3' miRNA: 3'- aCGUUau-----CGCG--AUGCGuuAUCGGGGa -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 34372 | 0.68 | 0.903404 |
Target: 5'- gGCAAgAGCgGCaagcgGCGCuccgAGCCCCUg -3' miRNA: 3'- aCGUUaUCG-CGa----UGCGuua-UCGGGGA- -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 113035 | 0.69 | 0.889774 |
Target: 5'- gGCGAUAcacCGCcagAC-CAAUAGCCCCUa -3' miRNA: 3'- aCGUUAUc--GCGa--UGcGUUAUCGGGGA- -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 114908 | 0.69 | 0.882586 |
Target: 5'- gGCAAUGGauccgGCGCcGUAGUCCCUg -3' miRNA: 3'- aCGUUAUCgcga-UGCGuUAUCGGGGA- -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 41170 | 0.69 | 0.881854 |
Target: 5'- aGCGuUAGCGCUagcccuagcguuaGCGC--UAGCCCUa -3' miRNA: 3'- aCGUuAUCGCGA-------------UGCGuuAUCGGGGa -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 35729 | 0.7 | 0.859588 |
Target: 5'- cUGCcgGGUGGCGCUgcuggagcccgACGcCGAggccgAGCCCCUg -3' miRNA: 3'- -ACG--UUAUCGCGA-----------UGC-GUUa----UCGGGGA- -5' |
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2638 | 5' | -52.5 | NC_001491.2 | + | 35169 | 0.71 | 0.804985 |
Target: 5'- cGCAAgcuccUGGUGCUggACGCGGUcuccaccgaggauuGGCCCCa -3' miRNA: 3'- aCGUU-----AUCGCGA--UGCGUUA--------------UCGGGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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