Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2638 | 5' | -52.5 | NC_001491.2 | + | 20260 | 0.66 | 0.963581 |
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Target: 5'- gGCAGUGGaaGCUgaggggguggACGUGGaAGCCCCUg -3' miRNA: 3'- aCGUUAUCg-CGA----------UGCGUUaUCGGGGA- -5' |
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| 2638 | 5' | -52.5 | NC_001491.2 | + | 81494 | 0.66 | 0.966971 |
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Target: 5'- gUGCAGcggagAGCGCcgACGCcgccacGCCCCa -3' miRNA: 3'- -ACGUUa----UCGCGa-UGCGuuau--CGGGGa -5' |
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| 2638 | 5' | -52.5 | NC_001491.2 | + | 60981 | 0.66 | 0.966971 |
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Target: 5'- cUGCGcgAGCGCUcugGCGUuuUGuucuacucGCCCCa -3' miRNA: 3'- -ACGUuaUCGCGA---UGCGuuAU--------CGGGGa -5' |
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| 2638 | 5' | -52.5 | NC_001491.2 | + | 32198 | 0.66 | 0.97307 |
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Target: 5'- gGCgGAUGGUGCcgGgGCgAGUAGCUCCUc -3' miRNA: 3'- aCG-UUAUCGCGa-UgCG-UUAUCGGGGA- -5' |
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| 2638 | 5' | -52.5 | NC_001491.2 | + | 16845 | 0.66 | 0.97307 |
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Target: 5'- cUGCGGuUGGCGCguggucaaguuuUACGCGAaggauaUAcGCCCCg -3' miRNA: 3'- -ACGUU-AUCGCG------------AUGCGUU------AU-CGGGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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