Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26384 | 3' | -55 | NC_005345.2 | + | 45330 | 0.65 | 0.741372 |
Target: 5'- -aGCCGGCG-AgCUGcUGCGCGGCc-- -3' miRNA: 3'- ugCGGCUGCaUgGAC-AUGCGCUGacu -5' |
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26384 | 3' | -55 | NC_005345.2 | + | 45564 | 0.73 | 0.342588 |
Target: 5'- -gGCCGACG-ACCcgcagGUGCGCGACa-- -3' miRNA: 3'- ugCGGCUGCaUGGa----CAUGCGCUGacu -5' |
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26384 | 3' | -55 | NC_005345.2 | + | 47466 | 0.69 | 0.555963 |
Target: 5'- cUGUCGGCG-ACCUGUGgGCGAUg-- -3' miRNA: 3'- uGCGGCUGCaUGGACAUgCGCUGacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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