Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2639 | 3' | -52.9 | NC_001491.2 | + | 11703 | 0.66 | 0.973774 |
Target: 5'- uUCUUCCGGGaGAGUGCUGGGGuCCGc -3' miRNA: 3'- gAGAGGGUCCgUUUAUGGUCUUcGGC- -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 31313 | 0.66 | 0.973774 |
Target: 5'- uUCUgCCUcGGCGGAgcucgGCgCGGAAGCCu -3' miRNA: 3'- gAGA-GGGuCCGUUUa----UG-GUCUUCGGc -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 26900 | 0.66 | 0.970937 |
Target: 5'- gUgUCCCAGGCAgcaccAGUACgcgCAGAagagcaugcGGCCGc -3' miRNA: 3'- gAgAGGGUCCGU-----UUAUG---GUCU---------UCGGC- -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 115716 | 0.66 | 0.970937 |
Target: 5'- gUCUCCCAGGUgu-UACUGGcgugcucGGCCa -3' miRNA: 3'- gAGAGGGUCCGuuuAUGGUCu------UCGGc -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 4864 | 0.66 | 0.970937 |
Target: 5'- gCUCUUCgGGGUGGcgGCCccAGcGGCCGg -3' miRNA: 3'- -GAGAGGgUCCGUUuaUGG--UCuUCGGC- -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 84765 | 0.66 | 0.967885 |
Target: 5'- ---cCCCGGGC-AGUGCCAaGGucguAGCCGc -3' miRNA: 3'- gagaGGGUCCGuUUAUGGU-CU----UCGGC- -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 6106 | 0.66 | 0.967885 |
Target: 5'- gCUCUcCCCGGGagaaGAAgcagAUC-GAAGCCGg -3' miRNA: 3'- -GAGA-GGGUCCg---UUUa---UGGuCUUCGGC- -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 142208 | 0.66 | 0.964613 |
Target: 5'- -cCUCCU-GGCAGG-GCCGGAAGCa- -3' miRNA: 3'- gaGAGGGuCCGUUUaUGGUCUUCGgc -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 86114 | 0.66 | 0.964613 |
Target: 5'- -gCUaCCAGGCcggcgauUACCAGcAGGCCGa -3' miRNA: 3'- gaGAgGGUCCGuuu----AUGGUC-UUCGGC- -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 3899 | 0.66 | 0.963588 |
Target: 5'- gUCUCUCGGGUAGgccAUguccgcguaggcgcGCCGGAGGCUc -3' miRNA: 3'- gAGAGGGUCCGUU---UA--------------UGGUCUUCGGc -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 71190 | 0.67 | 0.949205 |
Target: 5'- -cUUCCUgcguGGcGCGAAUACCGGucgguGGCCGg -3' miRNA: 3'- gaGAGGG----UC-CGUUUAUGGUCu----UCGGC- -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 42159 | 0.68 | 0.935091 |
Target: 5'- gUCUCCCcGGCGccaGGUGagaCAGGAGCUc -3' miRNA: 3'- gAGAGGGuCCGU---UUAUg--GUCUUCGGc -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 37261 | 0.68 | 0.924982 |
Target: 5'- uCUCUCUCGGGCGcGggcccgugacccuugACCAGAuauGGCCc -3' miRNA: 3'- -GAGAGGGUCCGUuUa--------------UGGUCU---UCGGc -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 137842 | 0.69 | 0.900066 |
Target: 5'- gUgUCCggagCAGGCugcAUGCCAGAAGUCGc -3' miRNA: 3'- gAgAGG----GUCCGuu-UAUGGUCUUCGGC- -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 32746 | 0.69 | 0.900066 |
Target: 5'- aCUCaUCCCAGGC---UACCGGGgcGGCgGc -3' miRNA: 3'- -GAG-AGGGUCCGuuuAUGGUCU--UCGgC- -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 84459 | 0.69 | 0.886404 |
Target: 5'- gCUCaccgcgCCCAGGCGA--GCgCAG-AGCCGg -3' miRNA: 3'- -GAGa-----GGGUCCGUUuaUG-GUCuUCGGC- -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 32688 | 0.69 | 0.879214 |
Target: 5'- ---aCCCAGGCAccgGCCgaGGAGGCCc -3' miRNA: 3'- gagaGGGUCCGUuuaUGG--UCUUCGGc -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 49270 | 0.72 | 0.737398 |
Target: 5'- gUCUCCCAGGCGGcgGCagaCGGGAGUa- -3' miRNA: 3'- gAGAGGGUCCGUUuaUG---GUCUUCGgc -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 32809 | 0.72 | 0.727301 |
Target: 5'- ---cCCCGGGCGucUACCAGcgcGAGCCGc -3' miRNA: 3'- gagaGGGUCCGUuuAUGGUC---UUCGGC- -5' |
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2639 | 3' | -52.9 | NC_001491.2 | + | 4787 | 0.72 | 0.727301 |
Target: 5'- -cCUCCCGGGCG---GCUGGGAGCgGg -3' miRNA: 3'- gaGAGGGUCCGUuuaUGGUCUUCGgC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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