Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26394 | 5' | -57.1 | NC_005345.2 | + | 22579 | 0.66 | 0.590603 |
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Target: 5'- cGAUCaGcAGAGGGGcGAgCCCcgGGCGu -3' miRNA: 3'- -CUGG-CaUCUCCCCaCUaGGGuaCCGU- -5' |
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| 26394 | 5' | -57.1 | NC_005345.2 | + | 40782 | 0.66 | 0.579655 |
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Target: 5'- cGGCCGgcGcGGGcGUGucgcguuccguGUCCCAgUGGCAc -3' miRNA: 3'- -CUGGCauCuCCC-CAC-----------UAGGGU-ACCGU- -5' |
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| 26394 | 5' | -57.1 | NC_005345.2 | + | 5456 | 0.66 | 0.575288 |
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Target: 5'- cGGCCGUAGGGGcGGUGccgagugaccgaCCCGaaaugGGCGg -3' miRNA: 3'- -CUGGCAUCUCC-CCACua----------GGGUa----CCGU- -5' |
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| 26394 | 5' | -57.1 | NC_005345.2 | + | 23942 | 0.66 | 0.536379 |
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Target: 5'- uGACCGgccGGGGGUGAUUcaCCGcgGGCu -3' miRNA: 3'- -CUGGCaucUCCCCACUAG--GGUa-CCGu -5' |
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| 26394 | 5' | -57.1 | NC_005345.2 | + | 33359 | 0.67 | 0.504673 |
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Target: 5'- cGGCgCG-AGAGGGGUGGgggCgGUGGCGc -3' miRNA: 3'- -CUG-GCaUCUCCCCACUag-GgUACCGU- -5' |
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| 26394 | 5' | -57.1 | NC_005345.2 | + | 2177 | 0.67 | 0.48399 |
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Target: 5'- uGACCGUGGc-GGGUGcaacgCCgAUGGCGc -3' miRNA: 3'- -CUGGCAUCucCCCACua---GGgUACCGU- -5' |
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| 26394 | 5' | -57.1 | NC_005345.2 | + | 7952 | 0.68 | 0.443908 |
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Target: 5'- aGCCGgcGGGcGGG-GAUCCCGUcGGUc -3' miRNA: 3'- cUGGCauCUC-CCCaCUAGGGUA-CCGu -5' |
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| 26394 | 5' | -57.1 | NC_005345.2 | + | 34732 | 0.7 | 0.344199 |
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Target: 5'- cGCCGUAuaccGcGGGGUGAUCCuCGcGGCGg -3' miRNA: 3'- cUGGCAU----CuCCCCACUAGG-GUaCCGU- -5' |
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| 26394 | 5' | -57.1 | NC_005345.2 | + | 31055 | 1.08 | 0.000628 |
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Target: 5'- aGACCGUAGAGGGGUGAUCCCAUGGCAc -3' miRNA: 3'- -CUGGCAUCUCCCCACUAGGGUACCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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