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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
264 | 3' | -51.4 | AC_000008.1 | + | 16983 | 0.66 | 0.735533 |
Target: 5'- -cGGGCAaaCUGCACCAGCcCGGgGc -3' miRNA: 3'- caCCCGUagGAUGUGGUUGuGUUgUu -5' |
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264 | 3' | -51.4 | AC_000008.1 | + | 23801 | 0.67 | 0.712478 |
Target: 5'- cUGGGUGUgCgcgGCACCAGCGCGu--- -3' miRNA: 3'- cACCCGUAgGa--UGUGGUUGUGUuguu -5' |
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264 | 3' | -51.4 | AC_000008.1 | + | 8505 | 0.68 | 0.653464 |
Target: 5'- -cGGGacuuUCCUgguACACCAGgGCAGCGGg -3' miRNA: 3'- caCCCgu--AGGA---UGUGGUUgUGUUGUU- -5' |
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264 | 3' | -51.4 | AC_000008.1 | + | 12875 | 0.7 | 0.489738 |
Target: 5'- uGUGGuGCAgaucUCCaACACCGACGCAAgGu -3' miRNA: 3'- -CACC-CGU----AGGaUGUGGUUGUGUUgUu -5' |
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264 | 3' | -51.4 | AC_000008.1 | + | 21268 | 1.06 | 0.00183 |
Target: 5'- gGUGGGCAUCCUACACCAACACAACAAc -3' miRNA: 3'- -CACCCGUAGGAUGUGGUUGUGUUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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