Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2640 | 5' | -57.5 | NC_001491.2 | + | 8852 | 0.66 | 0.828962 |
Target: 5'- aGUGCUG-GCAGUCCcUCCug-CAGCu -3' miRNA: 3'- aCACGAUaCGUCGGGcGGGugaGUCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 115695 | 0.66 | 0.823865 |
Target: 5'- gGUGUUGUGCuGGCCUGgauggucuCCCagguguuacuggcguGCUCGGCc -3' miRNA: 3'- aCACGAUACG-UCGGGC--------GGG---------------UGAGUCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 132178 | 0.66 | 0.820431 |
Target: 5'- --cGCgcaggGCGGCUaCGCCCACacCAGCu -3' miRNA: 3'- acaCGaua--CGUCGG-GCGGGUGa-GUCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 79202 | 0.66 | 0.811728 |
Target: 5'- gGUGCgc-GCGGCCCGCuCCAgggaggaGGCc -3' miRNA: 3'- aCACGauaCGUCGGGCG-GGUgag----UCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 111047 | 0.66 | 0.793837 |
Target: 5'- gUGUGCgccucagAUaGCAGCgCUGCCCACgccccCAGa -3' miRNA: 3'- -ACACGa------UA-CGUCG-GGCGGGUGa----GUCg -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 34796 | 0.66 | 0.793837 |
Target: 5'- --aGCUG-GCGGCCagcgGCCCGCUgAGa -3' miRNA: 3'- acaCGAUaCGUCGGg---CGGGUGAgUCg -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 39171 | 0.67 | 0.74669 |
Target: 5'- -aUGUUAgcgGCAGCCUGaauaCCACcgaUCAGCc -3' miRNA: 3'- acACGAUa--CGUCGGGCg---GGUG---AGUCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 136878 | 0.68 | 0.727057 |
Target: 5'- aGUGCggacGCGGCaUCGCUCGCgugugCGGCa -3' miRNA: 3'- aCACGaua-CGUCG-GGCGGGUGa----GUCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 27220 | 0.68 | 0.717111 |
Target: 5'- cGUGCUGgucggGUauAGCCCGUCCGCgcccGCc -3' miRNA: 3'- aCACGAUa----CG--UCGGGCGGGUGagu-CG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 27103 | 0.68 | 0.686873 |
Target: 5'- --cGCgg-GCGGCCCgGCgCCGCUCcggAGCg -3' miRNA: 3'- acaCGauaCGUCGGG-CG-GGUGAG---UCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 62578 | 0.68 | 0.685856 |
Target: 5'- -cUGCUguccGUGCGGCCCaaucCCCACaaccaagUCGGCg -3' miRNA: 3'- acACGA----UACGUCGGGc---GGGUG-------AGUCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 132911 | 0.69 | 0.635675 |
Target: 5'- --aGCUGUGCgcaAGCCaCGugaaCCCACUgCAGCg -3' miRNA: 3'- acaCGAUACG---UCGG-GC----GGGUGA-GUCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 26931 | 0.69 | 0.625394 |
Target: 5'- --aGC-AUGCGG-CCGCUgACUCGGCg -3' miRNA: 3'- acaCGaUACGUCgGGCGGgUGAGUCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 10193 | 0.71 | 0.530992 |
Target: 5'- gGUcGCUAcGCGGCCaGCCCcaugcccgucuuccACUCGGCg -3' miRNA: 3'- aCA-CGAUaCGUCGGgCGGG--------------UGAGUCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 81335 | 0.72 | 0.474724 |
Target: 5'- --aGCUGguUGCGGCCCGCcauagagCCGCcgCAGCg -3' miRNA: 3'- acaCGAU--ACGUCGGGCG-------GGUGa-GUCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 2411 | 0.72 | 0.456939 |
Target: 5'- gGUGCauccagGCGGCCCuCCguCUCAGCg -3' miRNA: 3'- aCACGaua---CGUCGGGcGGguGAGUCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 117990 | 0.74 | 0.394709 |
Target: 5'- gUGUGCaagagGguGCCCGCaCCaacaACUCGGCg -3' miRNA: 3'- -ACACGaua--CguCGGGCG-GG----UGAGUCG- -5' |
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2640 | 5' | -57.5 | NC_001491.2 | + | 129511 | 1.1 | 0.00134 |
Target: 5'- gUGUGCUAUGCAGCCCGCCCACUCAGCc -3' miRNA: 3'- -ACACGAUACGUCGGGCGGGUGAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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