Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26404 | 5' | -53.2 | NC_005345.2 | + | 19536 | 0.66 | 0.76253 |
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Target: 5'- cGGCAGC-CCCaUCGAGgacaggaCCGUGUUCa- -3' miRNA: 3'- -CUGUUGaGGG-AGUUCg------GGCACAAGcu -5' |
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| 26404 | 5' | -53.2 | NC_005345.2 | + | 46035 | 0.67 | 0.741324 |
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Target: 5'- gGGCGGC-CCCUCGguauguccggGGCCCGaGggUCGAu -3' miRNA: 3'- -CUGUUGaGGGAGU----------UCGGGCaCa-AGCU- -5' |
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| 26404 | 5' | -53.2 | NC_005345.2 | + | 4282 | 0.67 | 0.741324 |
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Target: 5'- gGGCAGCUCgggCUCGGGCUgCG-GUUCGAc -3' miRNA: 3'- -CUGUUGAGg--GAGUUCGG-GCaCAAGCU- -5' |
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| 26404 | 5' | -53.2 | NC_005345.2 | + | 35159 | 0.68 | 0.641572 |
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Target: 5'- gGGCAugGCUguggaagucggCgCUCAAGCCCGcGUUCGAc -3' miRNA: 3'- -CUGU--UGA-----------GgGAGUUCGGGCaCAAGCU- -5' |
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| 26404 | 5' | -53.2 | NC_005345.2 | + | 32074 | 0.7 | 0.540928 |
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Target: 5'- cGACcucgGGCUCggCCUCGGGCUCGgGUUCGAc -3' miRNA: 3'- -CUG----UUGAG--GGAGUUCGGGCaCAAGCU- -5' |
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| 26404 | 5' | -53.2 | NC_005345.2 | + | 34958 | 0.7 | 0.530005 |
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Target: 5'- gGACGACgaCgCUCAAGCCgGgGUUCGAc -3' miRNA: 3'- -CUGUUGa-GgGAGUUCGGgCaCAAGCU- -5' |
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| 26404 | 5' | -53.2 | NC_005345.2 | + | 10022 | 0.71 | 0.466411 |
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Target: 5'- aGCGACUCCCaCAGGUucgCCGUGU-CGAg -3' miRNA: 3'- cUGUUGAGGGaGUUCG---GGCACAaGCU- -5' |
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| 26404 | 5' | -53.2 | NC_005345.2 | + | 5390 | 0.74 | 0.327339 |
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Target: 5'- gGGCGAgUCCaCUCAGGCCCGgcggUCGGc -3' miRNA: 3'- -CUGUUgAGG-GAGUUCGGGCaca-AGCU- -5' |
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| 26404 | 5' | -53.2 | NC_005345.2 | + | 35365 | 0.8 | 0.138092 |
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Target: 5'- aACAGCgUCCUCAAGCCCGcGUUCGGc -3' miRNA: 3'- cUGUUGaGGGAGUUCGGGCaCAAGCU- -5' |
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| 26404 | 5' | -53.2 | NC_005345.2 | + | 35499 | 1.09 | 0.001492 |
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Target: 5'- gGACAACUCCCUCAAGCCCGUGUUCGAc -3' miRNA: 3'- -CUGUUGAGGGAGUUCGGGCACAAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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