Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26408 | 5' | -56.3 | NC_005345.2 | + | 44390 | 0.66 | 0.591891 |
Target: 5'- -gGCGAGGGUgCC-GUCgA-GGUCCGa -3' miRNA: 3'- aaCGCUCCCAaGGuCAGgUgCUAGGC- -5' |
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26408 | 5' | -56.3 | NC_005345.2 | + | 32955 | 0.66 | 0.591891 |
Target: 5'- -cGCGAGGcGgacgCCGG-CCGCG-UCCGc -3' miRNA: 3'- aaCGCUCC-Caa--GGUCaGGUGCuAGGC- -5' |
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26408 | 5' | -56.3 | NC_005345.2 | + | 31082 | 0.69 | 0.434801 |
Target: 5'- -cGCGAGGGaUUCCAgcuGUCCACcacCCGc -3' miRNA: 3'- aaCGCUCCC-AAGGU---CAGGUGcuaGGC- -5' |
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26408 | 5' | -56.3 | NC_005345.2 | + | 46231 | 0.7 | 0.397006 |
Target: 5'- cUGCGuuGGGccgcugcCCGGUCCGCcGAUCCGc -3' miRNA: 3'- aACGCu-CCCaa-----GGUCAGGUG-CUAGGC- -5' |
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26408 | 5' | -56.3 | NC_005345.2 | + | 43357 | 0.7 | 0.387893 |
Target: 5'- -gGCGAGGGggugCCGGUCgACGcguacgCCGa -3' miRNA: 3'- aaCGCUCCCaa--GGUCAGgUGCua----GGC- -5' |
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26408 | 5' | -56.3 | NC_005345.2 | + | 22361 | 0.73 | 0.248371 |
Target: 5'- cUGCGAcGcGGcgCCGG-CCACGAUCCGc -3' miRNA: 3'- aACGCU-C-CCaaGGUCaGGUGCUAGGC- -5' |
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26408 | 5' | -56.3 | NC_005345.2 | + | 36082 | 0.74 | 0.21206 |
Target: 5'- -gGCGGGGGaugggUCCGGUCCacACGAUgCCGc -3' miRNA: 3'- aaCGCUCCCa----AGGUCAGG--UGCUA-GGC- -5' |
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26408 | 5' | -56.3 | NC_005345.2 | + | 37057 | 1.07 | 0.000965 |
Target: 5'- cUUGCGAGGGUUCCAGUCCACGAUCCGa -3' miRNA: 3'- -AACGCUCCCAAGGUCAGGUGCUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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