Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26409 | 5' | -55.1 | NC_005345.2 | + | 38388 | 0.66 | 0.716441 |
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Target: 5'- uCGACgGCCggCaGGGGCUCGGcgucGGCg -3' miRNA: 3'- -GCUGaCGGaaGcCCUUGAGCUa---CCGa -5' |
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| 26409 | 5' | -55.1 | NC_005345.2 | + | 37950 | 0.66 | 0.69471 |
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Target: 5'- cCGACggUGCCcgagcggaUCGGGAuC-CGGUGGCUc -3' miRNA: 3'- -GCUG--ACGGa-------AGCCCUuGaGCUACCGA- -5' |
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| 26409 | 5' | -55.1 | NC_005345.2 | + | 35765 | 0.66 | 0.69471 |
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Target: 5'- cCGGCgggGCggggCGGuGggUUCGGUGGCUc -3' miRNA: 3'- -GCUGa--CGgaa-GCC-CuuGAGCUACCGA- -5' |
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| 26409 | 5' | -55.1 | NC_005345.2 | + | 18794 | 0.67 | 0.650598 |
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Target: 5'- gGGCUGCCgUCGGGcaggguGACggcggCGcgGGCc -3' miRNA: 3'- gCUGACGGaAGCCC------UUGa----GCuaCCGa -5' |
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| 26409 | 5' | -55.1 | NC_005345.2 | + | 35555 | 0.68 | 0.569822 |
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Target: 5'- gGGCcGCCUUCGGGcucgcccgggacgcGAuCUCGAaGGCg -3' miRNA: 3'- gCUGaCGGAAGCCC--------------UU-GAGCUaCCGa -5' |
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| 26409 | 5' | -55.1 | NC_005345.2 | + | 14855 | 0.68 | 0.562167 |
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Target: 5'- gGGCUGCag-CGGGcGCUCGAcgcGGCc -3' miRNA: 3'- gCUGACGgaaGCCCuUGAGCUa--CCGa -5' |
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| 26409 | 5' | -55.1 | NC_005345.2 | + | 11744 | 0.69 | 0.52971 |
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Target: 5'- uCGGCUcgccGCCUUCGucGGGCUCGGuuUGGCg -3' miRNA: 3'- -GCUGA----CGGAAGCc-CUUGAGCU--ACCGa -5' |
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| 26409 | 5' | -55.1 | NC_005345.2 | + | 21780 | 0.71 | 0.39922 |
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Target: 5'- gGGCgaGCCggggUCGGGuggacuGAUUCGAUGGCg -3' miRNA: 3'- gCUGa-CGGa---AGCCC------UUGAGCUACCGa -5' |
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| 26409 | 5' | -55.1 | NC_005345.2 | + | 6979 | 0.76 | 0.201896 |
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Target: 5'- gCGGCaggGCUUUCGGGGccGCugUCGAUGGCUg -3' miRNA: 3'- -GCUGa--CGGAAGCCCU--UG--AGCUACCGA- -5' |
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| 26409 | 5' | -55.1 | NC_005345.2 | + | 37232 | 1.08 | 0.001014 |
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Target: 5'- gCGACUGCCUUCGGGAACUCGAUGGCUg -3' miRNA: 3'- -GCUGACGGAAGCCCUUGAGCUACCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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