Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26414 | 3' | -58.2 | NC_005345.2 | + | 40684 | 0.66 | 0.515127 |
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Target: 5'- aGAACCccgaaacGGGAGUCGCgaccgcgCCCguGCUCGu -3' miRNA: 3'- -CUUGGaca----CCCUCAGCGa------GGG--CGAGU- -5' |
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| 26414 | 3' | -58.2 | NC_005345.2 | + | 22338 | 0.66 | 0.494413 |
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Target: 5'- -cACgUGUGGGggcgcgGGUCGCUgCCGCa-- -3' miRNA: 3'- cuUGgACACCC------UCAGCGAgGGCGagu -5' |
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| 26414 | 3' | -58.2 | NC_005345.2 | + | 48984 | 0.67 | 0.464081 |
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Target: 5'- -cGCgUGUgGGGAGcaCGCcggCCCGCUCGg -3' miRNA: 3'- cuUGgACA-CCCUCa-GCGa--GGGCGAGU- -5' |
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| 26414 | 3' | -58.2 | NC_005345.2 | + | 6137 | 0.67 | 0.425215 |
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Target: 5'- uGAACCUGcGGacccGGUCGCaCCgGCUCAu -3' miRNA: 3'- -CUUGGACaCCc---UCAGCGaGGgCGAGU- -5' |
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| 26414 | 3' | -58.2 | NC_005345.2 | + | 5935 | 0.68 | 0.388355 |
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Target: 5'- uGACCUGUGGGuGUgGCUgcggaCCggGCUCAu -3' miRNA: 3'- cUUGGACACCCuCAgCGAg----GG--CGAGU- -5' |
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| 26414 | 3' | -58.2 | NC_005345.2 | + | 12853 | 0.69 | 0.321206 |
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Target: 5'- aGGACCggugacgucGUGGGGGUgGCUCgccgccggguggCCGCUCGc -3' miRNA: 3'- -CUUGGa--------CACCCUCAgCGAG------------GGCGAGU- -5' |
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| 26414 | 3' | -58.2 | NC_005345.2 | + | 16791 | 0.7 | 0.305836 |
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Target: 5'- uGAAgCUGUGGGAGaugCGCUCguaCGCUgAg -3' miRNA: 3'- -CUUgGACACCCUCa--GCGAGg--GCGAgU- -5' |
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| 26414 | 3' | -58.2 | NC_005345.2 | + | 10769 | 0.7 | 0.276803 |
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Target: 5'- -uGCCUcccGGGAGUUGCUgCCGCUUg -3' miRNA: 3'- cuUGGAca-CCCUCAGCGAgGGCGAGu -5' |
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| 26414 | 3' | -58.2 | NC_005345.2 | + | 39132 | 1.08 | 0.000493 |
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Target: 5'- cGAACCUGUGGGAGUCGCUCCCGCUCAc -3' miRNA: 3'- -CUUGGACACCCUCAGCGAGGGCGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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