Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26416 | 5' | -56.3 | NC_005345.2 | + | 38773 | 0.66 | 0.626459 |
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Target: 5'- cGUGGGgCAccgacgCCCUCGAagGC-UCGUa -3' miRNA: 3'- -CACCCgGUca----GGGAGCUuaCGuAGCA- -5' |
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| 26416 | 5' | -56.3 | NC_005345.2 | + | 43704 | 0.66 | 0.626459 |
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Target: 5'- cGUGGGCCGGccucgaCCUCGAuacgaacGcCGUCGUc -3' miRNA: 3'- -CACCCGGUCag----GGAGCUua-----C-GUAGCA- -5' |
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| 26416 | 5' | -56.3 | NC_005345.2 | + | 39560 | 0.66 | 0.571161 |
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Target: 5'- -cGcGGCCGGUCCCggcCGGucGUGCGuUCGc -3' miRNA: 3'- caC-CCGGUCAGGGa--GCU--UACGU-AGCa -5' |
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| 26416 | 5' | -56.3 | NC_005345.2 | + | 16473 | 0.69 | 0.425739 |
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Target: 5'- -gGGGUCAgGUCgaccucggCCUCGAcgGCGUCGa -3' miRNA: 3'- caCCCGGU-CAG--------GGAGCUuaCGUAGCa -5' |
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| 26416 | 5' | -56.3 | NC_005345.2 | + | 13689 | 0.69 | 0.425739 |
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Target: 5'- -cGGGCagcucgCCCUCGAAUGCggCGa -3' miRNA: 3'- caCCCGguca--GGGAGCUUACGuaGCa -5' |
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| 26416 | 5' | -56.3 | NC_005345.2 | + | 36218 | 0.7 | 0.3617 |
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Target: 5'- gGUGGGCCAGUCCg-CGAGccUGCugcCGg -3' miRNA: 3'- -CACCCGGUCAGGgaGCUU--ACGua-GCa -5' |
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| 26416 | 5' | -56.3 | NC_005345.2 | + | 40295 | 1.07 | 0.00089 |
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Target: 5'- cGUGGGCCAGUCCCUCGAAUGCAUCGUc -3' miRNA: 3'- -CACCCGGUCAGGGAGCUUACGUAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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