miRNA: 3'- -AGGCG--UGUAG-UGUACCCU-------------GgCGUUGC- -5'
miRNA: 3'- aGG-CGUGuaGuGuaC-CCUGGCGUUGC- -5'
miRNA: 3'- aGGC-GUGUAGUGUaCCC-UGgCGUu--GC- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26423 | 3' | -54.5 | NC_005345.2 | + | 2517 | 0.7 | 0.499737 |
|
Target: 5'- uUCCGCugGCAUCgACGUGGGGgguuuccacgcggUgGCGACGg -3' miRNA: 3'- -AGGCG--UGUAG-UGUACCCU-------------GgCGUUGC- -5' |
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| 26423 | 3' | -54.5 | NC_005345.2 | + | 2047 | 0.66 | 0.709131 |
|
Target: 5'- cCCgGCGCggC-CcgGcGGGCCGCGGCGa -3' miRNA: 3'- aGG-CGUGuaGuGuaC-CCUGGCGUUGC- -5' |
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| 26423 | 3' | -54.5 | NC_005345.2 | + | 875 | 0.66 | 0.719965 |
|
Target: 5'- cCCGaCGCAUCGCA-GGGaACgGCGucuCGa -3' miRNA: 3'- aGGC-GUGUAGUGUaCCC-UGgCGUu--GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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