Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26425 | 3' | -52.3 | NC_005345.2 | + | 4666 | 0.67 | 0.805004 |
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Target: 5'- ---nCGGCCGUCGCCUucGCUGCu---- -3' miRNA: 3'- caacGCUGGCAGCGGA--UGAUGcaaga -5' |
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| 26425 | 3' | -52.3 | NC_005345.2 | + | 39918 | 0.67 | 0.774758 |
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Target: 5'- --cGCGGCguacgacuugagCGUCGCCgggACUACGUa-- -3' miRNA: 3'- caaCGCUG------------GCAGCGGa--UGAUGCAaga -5' |
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| 26425 | 3' | -52.3 | NC_005345.2 | + | 33787 | 0.68 | 0.753794 |
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Target: 5'- --cGCGcuGCCGUCGCCgcCUAC-UUCg -3' miRNA: 3'- caaCGC--UGGCAGCGGauGAUGcAAGa -5' |
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| 26425 | 3' | -52.3 | NC_005345.2 | + | 24721 | 0.68 | 0.743109 |
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Target: 5'- --cGCGACCGaacccUCGCCcgGCgugaacGCGUUCg -3' miRNA: 3'- caaCGCUGGC-----AGCGGa-UGa-----UGCAAGa -5' |
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| 26425 | 3' | -52.3 | NC_005345.2 | + | 4646 | 0.68 | 0.743109 |
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Target: 5'- ----gGGCCGUCGCCUGCUGUGccUCg -3' miRNA: 3'- caacgCUGGCAGCGGAUGAUGCa-AGa -5' |
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| 26425 | 3' | -52.3 | NC_005345.2 | + | 11905 | 0.69 | 0.676939 |
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Target: 5'- --cGCcGCCGUCGCCcgcUACUACGg--- -3' miRNA: 3'- caaCGcUGGCAGCGG---AUGAUGCaaga -5' |
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| 26425 | 3' | -52.3 | NC_005345.2 | + | 36154 | 0.72 | 0.48807 |
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Target: 5'- -gUGCGGCCGgcagCGCgaACUGCGUguUCa -3' miRNA: 3'- caACGCUGGCa---GCGgaUGAUGCA--AGa -5' |
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| 26425 | 3' | -52.3 | NC_005345.2 | + | 12590 | 0.73 | 0.426878 |
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Target: 5'- uGUUGCGGCCGcgaUCGUCgGCUACGcgCUc -3' miRNA: 3'- -CAACGCUGGC---AGCGGaUGAUGCaaGA- -5' |
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| 26425 | 3' | -52.3 | NC_005345.2 | + | 43756 | 1.09 | 0.001815 |
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Target: 5'- cGUUGCGACCGUCGCCUACUACGUUCUg -3' miRNA: 3'- -CAACGCUGGCAGCGGAUGAUGCAAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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