Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
26426 | 5' | -55.1 | NC_005345.2 | + | 12928 | 0.66 | 0.646335 |
Target: 5'- cGGCAgcaGGCUCGCg--GACUGgcccaccauuuGCAGGGc -3' miRNA: 3'- -CCGUg--UCGAGCGugaCUGAC-----------UGUCCU- -5' |
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26426 | 5' | -55.1 | NC_005345.2 | + | 3451 | 0.66 | 0.668616 |
Target: 5'- gGGCcCcgAGCUCGCGCgGGCUGuCGGa- -3' miRNA: 3'- -CCGuG--UCGAGCGUGaCUGACuGUCcu -5' |
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26426 | 5' | -55.1 | NC_005345.2 | + | 42553 | 0.66 | 0.679709 |
Target: 5'- cGGUccACAGCUCGUucgcCUG-C-GACAGGGg -3' miRNA: 3'- -CCG--UGUCGAGCGu---GACuGaCUGUCCU- -5' |
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26426 | 5' | -55.1 | NC_005345.2 | + | 16286 | 0.66 | 0.690755 |
Target: 5'- cGGCuGCAGCUUGUACUG-C-GGCAGc- -3' miRNA: 3'- -CCG-UGUCGAGCGUGACuGaCUGUCcu -5' |
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26426 | 5' | -55.1 | NC_005345.2 | + | 19246 | 0.66 | 0.701744 |
Target: 5'- aGGUuCGGCUCGCGCagGAC---CGGGAu -3' miRNA: 3'- -CCGuGUCGAGCGUGa-CUGacuGUCCU- -5' |
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26426 | 5' | -55.1 | NC_005345.2 | + | 26083 | 0.66 | 0.695159 |
Target: 5'- cGGCACGGCagacagcggcgaugUGCAC-GACcucGGCGGGAu -3' miRNA: 3'- -CCGUGUCGa-------------GCGUGaCUGa--CUGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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