Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26426 | 5' | -55.1 | NC_005345.2 | + | 36794 | 0.66 | 0.679709 |
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Target: 5'- cGGCACAGC-CGCAgCgcaGCUGACGa-- -3' miRNA: 3'- -CCGUGUCGaGCGU-Gac-UGACUGUccu -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 39849 | 0.69 | 0.48272 |
|
Target: 5'- cGGC-CGGC-CGCAC---CUGGCAGGAc -3' miRNA: 3'- -CCGuGUCGaGCGUGacuGACUGUCCU- -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 42553 | 0.66 | 0.679709 |
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Target: 5'- cGGUccACAGCUCGUucgcCUG-C-GACAGGGg -3' miRNA: 3'- -CCG--UGUCGAGCGu---GACuGaCUGUCCU- -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 44740 | 1.11 | 0.000593 |
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Target: 5'- uGGCACAGCUCGCACUGACUGACAGGAg -3' miRNA: 3'- -CCGUGUCGAGCGUGACUGACUGUCCU- -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 46017 | 0.66 | 0.679709 |
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Target: 5'- cGGCACGGCUgUGCAgUGGCgccucGGCGcGAa -3' miRNA: 3'- -CCGUGUCGA-GCGUgACUGa----CUGUcCU- -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 46670 | 0.68 | 0.524928 |
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Target: 5'- uGUACAucgagcucGCUCGCGCgcagGACUgGGCAGGc -3' miRNA: 3'- cCGUGU--------CGAGCGUGa---CUGA-CUGUCCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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