Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26426 | 5' | -55.1 | NC_005345.2 | + | 42553 | 0.66 | 0.679709 |
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Target: 5'- cGGUccACAGCUCGUucgcCUG-C-GACAGGGg -3' miRNA: 3'- -CCG--UGUCGAGCGu---GACuGaCUGUCCU- -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 3451 | 0.66 | 0.668616 |
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Target: 5'- gGGCcCcgAGCUCGCGCgGGCUGuCGGa- -3' miRNA: 3'- -CCGuG--UCGAGCGUGaCUGACuGUCcu -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 12928 | 0.66 | 0.646335 |
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Target: 5'- cGGCAgcaGGCUCGCg--GACUGgcccaccauuuGCAGGGc -3' miRNA: 3'- -CCGUg--UCGAGCGugaCUGAC-----------UGUCCU- -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 25584 | 0.67 | 0.61284 |
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Target: 5'- -cCGCAGCUCGgACgcGGCcGACAGGu -3' miRNA: 3'- ccGUGUCGAGCgUGa-CUGaCUGUCCu -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 16769 | 0.69 | 0.472422 |
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Target: 5'- -aCGCGGUgagCGCACUGuCcGACAGGGu -3' miRNA: 3'- ccGUGUCGa--GCGUGACuGaCUGUCCU- -5' |
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| 26426 | 5' | -55.1 | NC_005345.2 | + | 35751 | 0.66 | 0.701744 |
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Target: 5'- cGGCGCAGgaCGaACcGGCggGGCGGGGc -3' miRNA: 3'- -CCGUGUCgaGCgUGaCUGa-CUGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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